Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain

Aim: To maximize biomass production of an ochratoxigenic mould-controlling strain of Lachancea thermotolerans employing response surface methodology (RSM). Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pelinski, R., Cerrutti, P., Ponsone, M.L., Chulze, S., Galvagno, M.
Formato: JOUR
Lenguaje:English
Materias:
Biocontrol agent
Cane molasses
Experimental designs
Fermentation optimization
Lachancea thermotolerans
molasses
sugar
biocontrol agent
biomass
bioreactor
experimental design
fermentation
optimization
sugar cane
sustainability
temperature tolerance
yeast
article
batch fermentation
biological pest control
culture medium
culture optimization
dry weight
fungal strain
mould
nonhuman
productivity
response surface method
sugarcane
Antibiosis
Biological Control Agents
Biomass
Culture Media
Fermentation
Fungi
Statistics as Topic
Yeasts
Lachancea
Saccharum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02668254_v54_n5_p377_Pelinski
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Aim: To maximize biomass production of an ochratoxigenic mould-controlling strain of Lachancea thermotolerans employing response surface methodology (RSM). Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (gl -1): fermentable sugars (FS), 139·2, provided by sugar cane molasses (CMz), (NH 4) 2HPO 4 (DAP), 9·0, and yeast extract (YE), 2·5, was formulated. Maximal cell concentration obtained after 24h at 28°C was 24·2gl -1cell dry weight (CDW). The mathematical model obtained was validated in experiments performed in shaken-flask cultures and also in aerated bioreactors. Maximum yield and productivity values achieved were, respectively, of 0·23g CDW/g FS in a medium containing (gl -1): FS, 87·0; DAP, 7·0; YE, 1·0; and of 0·96g CDW l -1 h -1 in a medium containing (gl -1): FS, 150·8 plus DAP, 6·9. Conclusions: Optimized culture conditions for maximizing yeast biomass production determined in flask cultures were applicable at a larger scale. The highest yield values were attained in media containing relatively low-CMz concentrations supplemented with DAP and YE. Yeast extract would not be necessary if higher productivity is the aim. Significance and Impact of the Study: Cells of L. thermotolerans produced aerobically could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources. Response surface methodology allowed the fine-tuning of cultural conditions. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.

Ejemplares similares