DNA damage in workers occupationally exposed to pesticide mixtures

Pesticides are used in agriculture to protect crops but represent at the same time a potential risk to farmers and environment. The aim of this work is the evaluation of 54 subjects occupationally exposed to pesticides and 30 subjects as a control group using the quantification of DNA damage level b...

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Autores principales:	Simoniello, M.F., Kleinsorge, E.C., Scagnetti, J.A., Grigolato, R.A., Poletta, G.L., Carballo, M.A.
Formato:	JOUR
Materias:	Comet assay Farmworkers Genotoxicity Human biomonitoring Occupational exposure Pesticide applicators agricultural chemical carcinogen pesticide adult alcohol consumption Argentina article control group controlled study cultivated land developing country DNA damage environmental exposure female genotoxicity health hazard human industrial worker major clinical study male neoplasm occupational exposure priority journal protective equipment smoking Adult Agricultural Workers' Diseases Cell Survival Comet Assay Complex Mixtures DNA Damage Female Fluorescent Dyes Humans Life Style Male Middle Aged Occupational Exposure Pesticides Risk
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0260437X_v28_n8_p957_Simoniello
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0260437X_v28_n8_p957_Simoniello2023-10-03T15:11:51Z DNA damage in workers occupationally exposed to pesticide mixtures Simoniello, M.F. Kleinsorge, E.C. Scagnetti, J.A. Grigolato, R.A. Poletta, G.L. Carballo, M.A. Comet assay Farmworkers Genotoxicity Human biomonitoring Occupational exposure Pesticide applicators agricultural chemical carcinogen pesticide adult alcohol consumption Argentina article control group controlled study cultivated land developing country DNA damage environmental exposure female genotoxicity health hazard human industrial worker major clinical study male neoplasm occupational exposure priority journal protective equipment smoking Adult Agricultural Workers' Diseases Argentina Cell Survival Comet Assay Complex Mixtures DNA Damage Female Fluorescent Dyes Humans Life Style Male Middle Aged Occupational Exposure Pesticides Risk Pesticides are used in agriculture to protect crops but represent at the same time a potential risk to farmers and environment. The aim of this work is the evaluation of 54 subjects occupationally exposed to pesticides and 30 subjects as a control group using the quantification of DNA damage level by means of the alkaline Comet assay and the evaluation of repair processes. Damage index Comet assay (DICA) and damage index repair assay (DIRA) were studied in 27 pesticide applicator workers, 27 non-pesticide applicators and controls. Our results show that both exposed groups revealed significant increase in DICA when compared with controls (P < 0.0001), as well as in DIRA (P < 0.0001). However, the spraying group exhibited a marginally significant difference in DICA (P = 0.05) when years of exposure are considered and a significant difference (P < 0.05) when the personal protective equipment used by individuals was taken as a comparison factor. The influence of confounding factors on the genotoxic effects of occupational exposure to pesticides was investigated and no significant differences were observed considering age, gender, smoking and alcohol consumption in relation to DICA and DIRA. Since DNA damage is an important step in events leading from carcinogen exposure to cancer disease, our study highlights the potential health risk associated with agrochemical exposure in developing countries with vast cultivated areas, such as Argentina. Copyright © 2008 John Wiley & Sons, Ltd. Fil:Poletta, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0260437X_v28_n8_p957_Simoniello
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Comet assay Farmworkers Genotoxicity Human biomonitoring Occupational exposure Pesticide applicators agricultural chemical carcinogen pesticide adult alcohol consumption Argentina article control group controlled study cultivated land developing country DNA damage environmental exposure female genotoxicity health hazard human industrial worker major clinical study male neoplasm occupational exposure priority journal protective equipment smoking Adult Agricultural Workers' Diseases Argentina Cell Survival Comet Assay Complex Mixtures DNA Damage Female Fluorescent Dyes Humans Life Style Male Middle Aged Occupational Exposure Pesticides Risk
spellingShingle	Comet assay Farmworkers Genotoxicity Human biomonitoring Occupational exposure Pesticide applicators agricultural chemical carcinogen pesticide adult alcohol consumption Argentina article control group controlled study cultivated land developing country DNA damage environmental exposure female genotoxicity health hazard human industrial worker major clinical study male neoplasm occupational exposure priority journal protective equipment smoking Adult Agricultural Workers' Diseases Argentina Cell Survival Comet Assay Complex Mixtures DNA Damage Female Fluorescent Dyes Humans Life Style Male Middle Aged Occupational Exposure Pesticides Risk Simoniello, M.F. Kleinsorge, E.C. Scagnetti, J.A. Grigolato, R.A. Poletta, G.L. Carballo, M.A. DNA damage in workers occupationally exposed to pesticide mixtures
topic_facet	Comet assay Farmworkers Genotoxicity Human biomonitoring Occupational exposure Pesticide applicators agricultural chemical carcinogen pesticide adult alcohol consumption Argentina article control group controlled study cultivated land developing country DNA damage environmental exposure female genotoxicity health hazard human industrial worker major clinical study male neoplasm occupational exposure priority journal protective equipment smoking Adult Agricultural Workers' Diseases Argentina Cell Survival Comet Assay Complex Mixtures DNA Damage Female Fluorescent Dyes Humans Life Style Male Middle Aged Occupational Exposure Pesticides Risk
description	Pesticides are used in agriculture to protect crops but represent at the same time a potential risk to farmers and environment. The aim of this work is the evaluation of 54 subjects occupationally exposed to pesticides and 30 subjects as a control group using the quantification of DNA damage level by means of the alkaline Comet assay and the evaluation of repair processes. Damage index Comet assay (DICA) and damage index repair assay (DIRA) were studied in 27 pesticide applicator workers, 27 non-pesticide applicators and controls. Our results show that both exposed groups revealed significant increase in DICA when compared with controls (P < 0.0001), as well as in DIRA (P < 0.0001). However, the spraying group exhibited a marginally significant difference in DICA (P = 0.05) when years of exposure are considered and a significant difference (P < 0.05) when the personal protective equipment used by individuals was taken as a comparison factor. The influence of confounding factors on the genotoxic effects of occupational exposure to pesticides was investigated and no significant differences were observed considering age, gender, smoking and alcohol consumption in relation to DICA and DIRA. Since DNA damage is an important step in events leading from carcinogen exposure to cancer disease, our study highlights the potential health risk associated with agrochemical exposure in developing countries with vast cultivated areas, such as Argentina. Copyright © 2008 John Wiley & Sons, Ltd.
format	JOUR
author	Simoniello, M.F. Kleinsorge, E.C. Scagnetti, J.A. Grigolato, R.A. Poletta, G.L. Carballo, M.A.
author_facet	Simoniello, M.F. Kleinsorge, E.C. Scagnetti, J.A. Grigolato, R.A. Poletta, G.L. Carballo, M.A.
author_sort	Simoniello, M.F.
title	DNA damage in workers occupationally exposed to pesticide mixtures
title_short	DNA damage in workers occupationally exposed to pesticide mixtures
title_full	DNA damage in workers occupationally exposed to pesticide mixtures
title_fullStr	DNA damage in workers occupationally exposed to pesticide mixtures
title_full_unstemmed	DNA damage in workers occupationally exposed to pesticide mixtures
title_sort	dna damage in workers occupationally exposed to pesticide mixtures
url	http://hdl.handle.net/20.500.12110/paper_0260437X_v28_n8_p957_Simoniello
work_keys_str_mv	AT simoniellomf dnadamageinworkersoccupationallyexposedtopesticidemixtures AT kleinsorgeec dnadamageinworkersoccupationallyexposedtopesticidemixtures AT scagnettija dnadamageinworkersoccupationallyexposedtopesticidemixtures AT grigolatora dnadamageinworkersoccupationallyexposedtopesticidemixtures AT polettagl dnadamageinworkersoccupationallyexposedtopesticidemixtures AT carballoma dnadamageinworkersoccupationallyexposedtopesticidemixtures
_version_	1807322700331352064

DNA damage in workers occupationally exposed to pesticide mixtures

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