Screening for and characterization of phospholipase A1 hypersecretory mutants of Tetrahymena thermophila

We have described a procedure for the isolation of mutants of Tetrahymena thermophila with hyperscretion of phospholipase A1 (PLA1). Using random chemical mutagenesis, uniparental cytogamy, genetic crossing and a new, fast and effective screening procedure, four PLA1-hypersecretory mutants were isol...

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Detalles Bibliográficos
Autores principales: Hartmann, M., Guberman, A., Florin-Christensen, M., Tiedtke, A.
Formato: JOUR
Materias:
lysosome enzyme
phosphatidylcholine
phospholipase A1
article
bacterium isolation
bacterium mutant
chemical mutagenesis
enzyme activity
enzyme analysis
enzyme release
fermentation
molecular cloning
nonhuman
screening
strain difference
supernatant
Tetrahymena thermophila
Bacteria (microorganisms)
Prokaryota
Protozoa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01757598_v54_n3_p390_Hartmann
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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