Detection of changes in mould cell wall stress-related gene expression by a novel reverse transcription real-time PCR method

The cell wall integrity (CWI) pathway is activated in response to cell wall stresses due to different food-related environments. Rho1 is one of the main regulators within such pathway. The objective of this work was to design an easy-to-use RT-qPCR technique for the evaluation of the Rho1 gene expre...

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Detalles Bibliográficos
Autores principales: da Cruz Cabral, L., Delgado, J., Andrade, M.J., Rodríguez, M., Rodríguez, A.
Formato: JOUR
Materias:
Cell wall integrity
Filamentous fungi
Rho1 gene
Stress
protein
protein PgAFP
unclassified drug
antifungal agent
fungal protein
primer DNA
Rho guanine nucleotide binding protein
Article
Aspergillus flavus
computer model
controlled study
fungal cell wall
fungal gene
fungus growth
gene expression
gene overexpression
genetic conservation
mould
nonhuman
nucleotide sequence
Penicillium
Penicillium chrysogenum
Penicillium polonucum
quantitative analysis
real time polymerase chain reaction
reverse transcription polymerase chain reaction
sensitivity and specificity
species
sporogenesis
wall stress
analysis
cell wall
food contamination
food control
gene expression regulation
genetics
metabolism
procedures
reverse transcription
Antifungal Agents
Base Sequence
Cell Wall
DNA Primers
Food Contamination
Food Microbiology
Fungal Proteins
Gene Expression
Gene Expression Regulation, Fungal
Real-Time Polymerase Chain Reaction
Reverse Transcription
rho GTP-Binding Proteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01681605_v275_n_p17_daCruzCabral
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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