Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina

Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction pro...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pimper, L.E., Remis, M.I., Goodall, R.N.P., Baker, C.S.
Formato: JOUR
Materias:
Bones
Cephalorhynchus commersonii
Commerson's dolphin
DNA extraction
Population genetic analysis
Teeth
biodegradation
biomarker
bone
DNA
dolphin
genetic analysis
population genetics
sex determination
tooth
Argentina
South America
Tierra del Fuego [(PRV) Argentina]
Cetacea
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01675427_v35_n3_p330_Pimper
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01675427_v35_n3_p330_Pimper
record_format dspace
spelling todo:paper_01675427_v35_n3_p330_Pimper2023-10-03T15:04:54Z Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina Pimper, L.E. Remis, M.I. Goodall, R.N.P. Baker, C.S. Bones Cephalorhynchus commersonii Commerson's dolphin DNA extraction Population genetic analysis Teeth biodegradation biomarker bone DNA dolphin genetic analysis population genetics sex determination tooth Argentina South America Tierra del Fuego [(PRV) Argentina] Cephalorhynchus commersonii Cetacea Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction process is presented here for 212 Commerson's dolphin (Cephalorhynchus commersonii) samples collected in Tierra del Fuego, Argentina, from 1974 to 2004. From the total samples, 82.5% (n = 175) allow for amplification to occur of a minimum length of 200 pb for the mtDNA control region, 66.5% (n = 141) of 400 bp, and 51% (n = 108) of 500 bp. A total of 11 haplotypes were defined from 10 polymorphic sites. From the initial number of samples, 64.6% could be sexed via molecular markers. This method is effective for processing large quantities of degraded samples over a short time period, analysing a representative number of mtDNA haplotypes, and allowing robust estimation of historical genetic diversity and trends over time. Fil:Pimper, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Remis, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01675427_v35_n3_p330_Pimper
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bones
Cephalorhynchus commersonii
Commerson's dolphin
DNA extraction
Population genetic analysis
Teeth
biodegradation
biomarker
bone
DNA
dolphin
genetic analysis
population genetics
sex determination
tooth
Argentina
South America
Tierra del Fuego [(PRV) Argentina]
Cephalorhynchus commersonii
Cetacea
spellingShingle Bones
Cephalorhynchus commersonii
Commerson's dolphin
DNA extraction
Population genetic analysis
Teeth
biodegradation
biomarker
bone
DNA
dolphin
genetic analysis
population genetics
sex determination
tooth
Argentina
South America
Tierra del Fuego [(PRV) Argentina]
Cephalorhynchus commersonii
Cetacea
Pimper, L.E.
Remis, M.I.
Goodall, R.N.P.
Baker, C.S.
Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
topic_facet Bones
Cephalorhynchus commersonii
Commerson's dolphin
DNA extraction
Population genetic analysis
Teeth
biodegradation
biomarker
bone
DNA
dolphin
genetic analysis
population genetics
sex determination
tooth
Argentina
South America
Tierra del Fuego [(PRV) Argentina]
Cephalorhynchus commersonii
Cetacea
description Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction process is presented here for 212 Commerson's dolphin (Cephalorhynchus commersonii) samples collected in Tierra del Fuego, Argentina, from 1974 to 2004. From the total samples, 82.5% (n = 175) allow for amplification to occur of a minimum length of 200 pb for the mtDNA control region, 66.5% (n = 141) of 400 bp, and 51% (n = 108) of 500 bp. A total of 11 haplotypes were defined from 10 polymorphic sites. From the initial number of samples, 64.6% could be sexed via molecular markers. This method is effective for processing large quantities of degraded samples over a short time period, analysing a representative number of mtDNA haplotypes, and allowing robust estimation of historical genetic diversity and trends over time.
format JOUR
author Pimper, L.E.
Remis, M.I.
Goodall, R.N.P.
Baker, C.S.
author_facet Pimper, L.E.
Remis, M.I.
Goodall, R.N.P.
Baker, C.S.
author_sort Pimper, L.E.
title Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
title_short Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
title_full Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
title_fullStr Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
title_full_unstemmed Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina
title_sort teeth and bones as sources of dna for genetic diversity and sex identification of commerson's dolphins (cephalorhynchus commersonii) from tierra del fuego, argentina
url http://hdl.handle.net/20.500.12110/paper_01675427_v35_n3_p330_Pimper
work_keys_str_mv AT pimperle teethandbonesassourcesofdnaforgeneticdiversityandsexidentificationofcommersonsdolphinscephalorhynchuscommersoniifromtierradelfuegoargentina
AT remismi teethandbonesassourcesofdnaforgeneticdiversityandsexidentificationofcommersonsdolphinscephalorhynchuscommersoniifromtierradelfuegoargentina
AT goodallrnp teethandbonesassourcesofdnaforgeneticdiversityandsexidentificationofcommersonsdolphinscephalorhynchuscommersoniifromtierradelfuegoargentina
AT bakercs teethandbonesassourcesofdnaforgeneticdiversityandsexidentificationofcommersonsdolphinscephalorhynchuscommersoniifromtierradelfuegoargentina
_version_ 1807323764138967040

Ejemplares similares