Purification and characterization of a soluble nucleoside diphosphate kinase in Trypanosoma cruzi

A soluble nucleoside diphosphate kinase (NDP kinase) was purified and characterized in epimastigote forms of Trypanosoma cruzi. The enzyme was purified by affinity chromatography on Blue-agarose and Q-Sepharose columns and by FPLC on a Superose 12 column. A membrane-associated NDP kinase was identif...

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Detalles Bibliográficos
Autores principales: Ulloa, R.M., Muschietti, J.P., Veron, M., Torres, H.N., Tellez-Iñón, M.T.
Formato: JOUR
Materias:
Nucleoside diphosphate kinase
Phosphoenzyme intermediate
Trypanosoma cruzi
nucleoside diphosphate kinase
article
controlled study
enzyme analysis
enzyme purification
immunoblotting
nonhuman
priority journal
trypanosoma cruzi
Adenosine Triphosphate
Animal
Cross Reactions
Guanosine Diphosphate
Kinetics
Nucleoside-Diphosphate Kinase
Phosphorylation
Protein Conformation
Protein Denaturation
Protozoan Proteins
Solubility
Species Specificity
Substrate Specificity
Support, Non-U.S. Gov't
Temperature
Thymine Nucleotides
Candida albicans
Dictyostelium discoideum
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01666851_v70_n1-2_p119_Ulloa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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