Evaluation of the proacrosin/acrosin system and its mechanism of activation in human sperm extracts

Acrosin is an acrosomal protease synthesized as a proenzyme and activated into β-acrosin during the acrosome reaction. In the present study, a set of sensitive assays was developed to identify the proacrosin/acrosin system and to evaluate its activation pattern in human sperm extracts. Immunocytoche...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zahn, A., Furlong, L.I., Biancotti, J.C., Ghiringhelli, P.D., Marín-Briggiler, C.I., Vazquez-Levin, M.H.
Formato:	JOUR
Materias:	Acrosin Activation Human spermatozoa Proteases acrosin benzamidine calcium chloride cell extract monoclonal antibody proacrosin acrosome amino acid sequence article controlled study enzyme activation enzyme activity enzyme assay human human cell immunocytochemistry male priority journal protein analysis sperm spermatozoon staining Western blotting zymography Amino Acid Sequence Animals Enzyme Activation Enzyme Precursors Humans Hydrogen-Ion Concentration Immunohistochemistry Male Molecular Sequence Data Molecular Weight Spermatozoa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01650378_v54_n1-2_p43_Zahn
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

Evaluation of the proacrosin/acrosin system and its mechanism of activation in human sperm extracts
Publicado: (2002)

Biochemical and molecular studies of the proacrosin/acrosin system in patients with unexplained infertility [4]
por: Marí, S.I., et al.

Biochemical and molecular studies of the proacrosin/acrosin system in patients with unexplained infertility [4]
Publicado: (2003)

Binding of recombinant human proacrosin/acrosin to zona pellucida glycoproteins. II. Participation of mannose residues in the interaction
por: Furlong, L.I., et al.

Binding of recombinant human proacrosin/acrosin to zona pellucida glycoproteins. II. Participation of mannose residues in the interaction
Publicado: (2005)

Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC
por: Furlong, L.I., et al.

Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC
Publicado: (2005)

Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
por: Prado Acosta, Mariano, et al.
Publicado: (2008)

Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
por: Acosta, M.P., et al.
Publicado: (2008)

Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
por: Acosta, M.P., et al.

Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356
por: Acosta, M.P., et al.
Publicado: (2008)

Studies on the catalytic activity of human hepatic porphobilinogen deaminase.
Publicado: (1997)

Studies on the catalytic activity of human hepatic porphobilinogen deaminase.
por: Mazzetti, M.B., et al.

A novel activating effect of the regulatory subunit of protein kinase A on catalytic subunit activity
por: Rinaldi, Jimena Julieta, et al.
Publicado: (2008)

A novel activating effect of the regulatory subunit of protein kinase A on catalytic subunit activity
por: Rinaldi, J., et al.

Sequence and structure of the rat housekeeping PBG-D isoform
Publicado: (1998)

Sequence and structure of the rat housekeeping PBG-D isoform
por: Cardalda, C.A., et al.

Characterization of the farnesyl pyrophosphate synthase of Trypanosoma cruzi by homology modeling and molecular dynamics
por: Sigman, Lucas, et al.
Publicado: (2006)

Characterization of the farnesyl pyrophosphate synthase of Trypanosoma cruzi by homology modeling and molecular dynamics
por: Sigman, L., et al.

Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites
Publicado: (2000)

Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites
por: Pereira, C.A., et al.

A two-stage model for lipid modulation of the activity of integral membrane proteins
por: Dodes Traian, M.M., et al.
Publicado: (2012)

A two-stage model for lipid modulation of the activity of integral membrane proteins
por: Dodes Traian, M.M., et al.

A two-stage model for lipid modulation of the activity of integral membrane proteins
por: Dodes Traian, M.M., et al.
Publicado: (2012)

A two-stage model for lipid modulation of the activity of integral membrane proteins
por: Levi, Valeria
Publicado: (2012)

Inhibitory effect of quercetin on matrix metalloproteinase 9 activity Molecular mechanism and structure-activity relationship of the flavonoid-enzyme interaction
por: Estrin, Dario Ariel, et al.
Publicado: (2010)

Inhibitory effect of quercetin on matrix metalloproteinase 9 activity Molecular mechanism and structure-activity relationship of the flavonoid-enzyme interaction
por: Saragusti, A.C., et al.

Activity and stability of hammerhead ribozymes containing 2′-C-methyluridine: A new RNA mimic
Publicado: (2005)

Activity and stability of hammerhead ribozymes containing 2′-C-methyluridine: A new RNA mimic
por: Gallo, M., et al.

Cysteine-rich positions outside the structural zinc motif of human papillomavirus E7 provide conformational modulation and suggest functional redox roles
por: Chemes, L.B., et al.

Immunolocalization of bovine sperm protease BSp120 by light and electron microscopy during capacitation and the acrosome reaction: Its role in in vitro fertilization
por: Cesari, A., et al.

A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
Publicado: (1996)

A putative pyruvate dehydrogenase α subunit gene from Trypanosoma cruzi
por: Buscaglia, C.A., et al.

Cysteine-rich positions outside the structural zinc motif of human papillomavirus E7 provide conformational modulation and suggest functional redox roles
por: Chemes, Lucía Beatriz, et al.
Publicado: (2014)

The crystal structure of the MAP kinase LmaMPK10 from leishmania major reveals parasite-specific features and regulatory mechanisms
por: Turjanski, Adrián Gustavo, et al.
Publicado: (2012)

The crystal structure of the MAP kinase LmaMPK10 from leishmania major reveals parasite-specific features and regulatory mechanisms
por: Horjales, S., et al.

Evidence of an essential lysine in pig liver 5-aminolevulinic acid dehydratase
por: Sopena de Kracoff, Yolanda Elvira, et al.
Publicado: (1995)

Evidence of an essential lysine in pig liver 5-aminolevulinic acid dehydratase
por: Sopena De Kracoff, Y.E., et al.

Immunolocalization of bovine sperm protease BSp120 by light and electron microscopy during capacitation and the acrosome reaction: Its role in in vitro fertilization
por: Vazquez, Mónica Hebe
Publicado: (2004)

Identification of a UPTG inhibitor protein from maize endosperm: high homology with sucrose synthase protein.
por: Wald, Flavia A., et al.
Publicado: (1998)