Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities

In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 μg...

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Detalles Bibliográficos
Autores principales: Chattopadhyay, K., Ghosh, T., Pujol, C.A., Carlucci, M.J., Damonte, E.B., Ray, B.
Formato: JOUR
Materias:
Anti-HSV activity
Galactan sulfate
Gracilaria corticata
antivirus agent
galactan
Gracilaria corticata extract
periodate
polysaccharide
unclassified drug
animal cell
anion exchange chromatography
anticoagulation
antiviral activity
article
controlled study
drug structure
drug sulfation
gel permeation chromatography
Gracilaria
Herpes simplex virus 1
Herpes simplex virus 2
IC 50
matrix assisted laser desorption ionization time of flight mass spectrometry
molecular weight
nonhuman
nuclear magnetic resonance spectroscopy
Vero cell
Animals
Anticoagulants
Antiviral Agents
Cercopithecus aethiops
Chromatography, Gel
Complex Mixtures
Galactans
Herpesvirus 1, Human
Herpesvirus 2, Human
Hydrolysis
Magnetic Resonance Spectroscopy
Molecular Weight
Oxidation-Reduction
Periodic Acid
Polysaccharides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sulfates
Vero Cells
Simplexvirus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01418130_v43_n4_p346_Chattopadhyay
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 μg/ml. Sub-fraction F3, which has a molecular mass of 30 kDa, consists of a backbone of β-(1 → 3) and α-(1 → 4)-linked-galactopyranosyl residues. This linear galactan contained Gal2Xyl1, Gal2AnGal2, Gal4 and Me-Gal3AnGal2 as oligomeric building subunits. Sulfate group was located at C-4 of (1 → 3)-linked galactopyranosyl residues of the native galactan, and appeared to be very important for the anti-herpetic activity. © 2008 Elsevier B.V. All rights reserved.

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