Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis

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We assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some...

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Autores principales: Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S.
Formato: JOUR
Materias:
Biomass
Cell culture
Escherichia coli
Mutagenesis
Strain
Synthesis (chemical)
ArcA mutants
Leaky mutation
Microaerobiosis
Poly(3-hydroxybutyrate) synthesis
Organic polymers
poly(3 hydroxybutyric acid)
bacteriology
gene
mutagenicity
recombination
aerobic metabolism
allele
arcA gene
arcA2 gene
article
Azotobacter
Azotobacter FA8
bacterial gene
bacterial growth
bacterial strain
biomass
bioreactor
dry weight
gene deletion
gene mutation
nonhuman
pha gene
phenotype
synthesis
wild type
Aerobiosis
Bacterial Outer Membrane Proteins
Culture Media
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Hydroxybutyrates
Mutation
Polyesters
Recombination, Genetic
Repressor Proteins
Arca
Bacteria (microorganisms)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00992240_v72_n4_p2614_Nikel
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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