Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor

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Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. legumin...

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Autores principales:	Bonomi, H.R., Posadas, D.M., Paris, G., Del Carmen Carrica, M., Frederickson, M., Pietrasanta, L.I., Bogomolni, R.A., Zorreguieta, A., Goldbaum, F.A.
Formato:	JOUR
Materias:	bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00278424_v109_n30_p12135_Bonomi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00278424_v109_n30_p12135_Bonomi2023-10-03T14:38:07Z Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor Bonomi, H.R. Posadas, D.M. Paris, G. Del Carmen Carrica, M. Frederickson, M. Pietrasanta, L.I. Bogomolni, R.A. Zorreguieta, A. Goldbaum, F.A. bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a peanodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00278424_v109_n30_p12135_Bonomi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum
spellingShingle	bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum Bonomi, H.R. Posadas, D.M. Paris, G. Del Carmen Carrica, M. Frederickson, M. Pietrasanta, L.I. Bogomolni, R.A. Zorreguieta, A. Goldbaum, F.A. Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
topic_facet	bacterial polysaccharide bacterial protein exopolysaccharide light oxygen and voltage histidine kinase unclassified drug adaptation amino terminal sequence article bacterial gene bacterium culture biofilm blue light environmental factor illumination light lov gene nodulation nonhuman pea plant root priority journal protein synthesis real time polymerase chain reaction reverse transcription polymerase chain reaction Rhizobium leguminosarum symbiosis Amino Acid Sequence Bacterial Adhesion Base Sequence Biofilms Blotting, Western Flagella Gentian Violet Light Microscopy, Electron, Scanning Molecular Sequence Data Peas Photoreceptors, Microbial Plant Root Nodulation Polysaccharides, Bacterial Protein Kinases Protein Structure, Tertiary Real-Time Polymerase Chain Reaction Rhizobium leguminosarum Sequence Alignment Sequence Analysis, DNA Statistics, Nonparametric Symbiosis algae Bacteria (microorganisms) Embryophyta Fabaceae Fungi Pisum sativum Rhizobium leguminosarum
description	Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are bluelight receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a peanodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum.
format	JOUR
author	Bonomi, H.R. Posadas, D.M. Paris, G. Del Carmen Carrica, M. Frederickson, M. Pietrasanta, L.I. Bogomolni, R.A. Zorreguieta, A. Goldbaum, F.A.
author_facet	Bonomi, H.R. Posadas, D.M. Paris, G. Del Carmen Carrica, M. Frederickson, M. Pietrasanta, L.I. Bogomolni, R.A. Zorreguieta, A. Goldbaum, F.A.
author_sort	Bonomi, H.R.
title	Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
title_short	Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
title_full	Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
title_fullStr	Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
title_full_unstemmed	Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor
title_sort	light regulates attachment, exopolysaccharide production, and nodulation in rhizobium leguminosarum through a lov-histidine kinase photoreceptor
url	http://hdl.handle.net/20.500.12110/paper_00278424_v109_n30_p12135_Bonomi
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Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor

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