Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland
The number of androgen (AR) and estrogen receptor (ER) binding sites was quantitated in cytosol fractions from adult intact male and female rats. The apparent equilibrium constants of dissociation were: 0.8 × 10−9 M for the AR measured with [3H]-R 1881 and 0.6 × 10−9 M for the ER labelled with [3H]-...
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todo:paper_00224731_v13_n11_p1331_Calandra2023-10-03T14:33:02Z Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland Calandra, R.S. Lüthy, I. Finocchiaro, L. Terrab, R. androgen receptor cytosol receptor estradiol estradiol h 3 estrogen receptor hormone receptor methyltrienolone h 3 metribolone radioisotope sex hormone unclassified drug adrenal gland animal experiment article endocrine system female genital system gonadectomy in vitro study male genital system orchiectomy ovariectomy preliminary communication rat sex difference sex differentiation Animalia The number of androgen (AR) and estrogen receptor (ER) binding sites was quantitated in cytosol fractions from adult intact male and female rats. The apparent equilibrium constants of dissociation were: 0.8 × 10−9 M for the AR measured with [3H]-R 1881 and 0.6 × 10−9 M for the ER labelled with [3H]-estradiol. The mean (±SD) number of available binding sites were (fmol/mg cytosol protein): male: AR: 88.6 ± 7.7 ER: 8.5 ± 1.4; female: AR: 23.8 ± 1.6 and ER: 7.6 ± 0.5. Orchidectomy Caused a two-fold increase in the number of available androgen and estrogen binding sites with a clear-cut reduction 10 days after castration. Ovariectomy elicited an unexpected decrease of ER one day after gonadectomy and full recovery of binding sites was found at 10 days. A significant increase in AR was seen 4 days following ovariectomy. © 1980, All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224731_v13_n11_p1331_Calandra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
androgen receptor cytosol receptor estradiol estradiol h 3 estrogen receptor hormone receptor methyltrienolone h 3 metribolone radioisotope sex hormone unclassified drug adrenal gland animal experiment article endocrine system female genital system gonadectomy in vitro study male genital system orchiectomy ovariectomy preliminary communication rat sex difference sex differentiation Animalia |
| spellingShingle |
androgen receptor cytosol receptor estradiol estradiol h 3 estrogen receptor hormone receptor methyltrienolone h 3 metribolone radioisotope sex hormone unclassified drug adrenal gland animal experiment article endocrine system female genital system gonadectomy in vitro study male genital system orchiectomy ovariectomy preliminary communication rat sex difference sex differentiation Animalia Calandra, R.S. Lüthy, I. Finocchiaro, L. Terrab, R. Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| topic_facet |
androgen receptor cytosol receptor estradiol estradiol h 3 estrogen receptor hormone receptor methyltrienolone h 3 metribolone radioisotope sex hormone unclassified drug adrenal gland animal experiment article endocrine system female genital system gonadectomy in vitro study male genital system orchiectomy ovariectomy preliminary communication rat sex difference sex differentiation Animalia |
| description |
The number of androgen (AR) and estrogen receptor (ER) binding sites was quantitated in cytosol fractions from adult intact male and female rats. The apparent equilibrium constants of dissociation were: 0.8 × 10−9 M for the AR measured with [3H]-R 1881 and 0.6 × 10−9 M for the ER labelled with [3H]-estradiol. The mean (±SD) number of available binding sites were (fmol/mg cytosol protein): male: AR: 88.6 ± 7.7 ER: 8.5 ± 1.4; female: AR: 23.8 ± 1.6 and ER: 7.6 ± 0.5. Orchidectomy Caused a two-fold increase in the number of available androgen and estrogen binding sites with a clear-cut reduction 10 days after castration. Ovariectomy elicited an unexpected decrease of ER one day after gonadectomy and full recovery of binding sites was found at 10 days. A significant increase in AR was seen 4 days following ovariectomy. © 1980, All rights reserved. |
| format |
JOUR |
| author |
Calandra, R.S. Lüthy, I. Finocchiaro, L. Terrab, R. |
| author_facet |
Calandra, R.S. Lüthy, I. Finocchiaro, L. Terrab, R. |
| author_sort |
Calandra, R.S. |
| title |
Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| title_short |
Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| title_full |
Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| title_fullStr |
Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| title_full_unstemmed |
Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| title_sort |
influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland |
| url |
http://hdl.handle.net/20.500.12110/paper_00224731_v13_n11_p1331_Calandra |
| work_keys_str_mv |
AT calandrars influenceofsexandgonadectomyonsexsteroidreceptorsinratadrenalgland AT luthyi influenceofsexandgonadectomyonsexsteroidreceptorsinratadrenalgland AT finocchiarol influenceofsexandgonadectomyonsexsteroidreceptorsinratadrenalgland AT terrabr influenceofsexandgonadectomyonsexsteroidreceptorsinratadrenalgland |
| _version_ |
1807319362994962432 |