Influence of sex and gonadectomy on sex steroid receptors in rat adrenal gland

The number of androgen (AR) and estrogen receptor (ER) binding sites was quantitated in cytosol fractions from adult intact male and female rats. The apparent equilibrium constants of dissociation were: 0.8 × 10−9 M for the AR measured with [3H]-R 1881 and 0.6 × 10−9 M for the ER labelled with [3H]-...

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Detalles Bibliográficos
Autores principales: Calandra, R.S., Lüthy, I., Finocchiaro, L., Terrab, R.
Formato: JOUR
Materias:
androgen receptor
cytosol receptor
estradiol
estradiol h 3
estrogen receptor
hormone receptor
methyltrienolone h 3
metribolone
radioisotope
sex hormone
unclassified drug
adrenal gland
animal experiment
article
endocrine system
female genital system
gonadectomy
in vitro study
male genital system
orchiectomy
ovariectomy
preliminary communication
rat
sex difference
sex differentiation
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224731_v13_n11_p1331_Calandra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The number of androgen (AR) and estrogen receptor (ER) binding sites was quantitated in cytosol fractions from adult intact male and female rats. The apparent equilibrium constants of dissociation were: 0.8 × 10−9 M for the AR measured with [3H]-R 1881 and 0.6 × 10−9 M for the ER labelled with [3H]-estradiol. The mean (±SD) number of available binding sites were (fmol/mg cytosol protein): male: AR: 88.6 ± 7.7 ER: 8.5 ± 1.4; female: AR: 23.8 ± 1.6 and ER: 7.6 ± 0.5. Orchidectomy Caused a two-fold increase in the number of available androgen and estrogen binding sites with a clear-cut reduction 10 days after castration. Ovariectomy elicited an unexpected decrease of ER one day after gonadectomy and full recovery of binding sites was found at 10 days. A significant increase in AR was seen 4 days following ovariectomy. © 1980, All rights reserved.

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