Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings

After the emergence of the Ceratitis capitata imago, the pale and folded wings are expanded and sclerotized to acquire the definitive form and to stabilize the cuticle. The wings of this fly show a specific pattern of brownish and black spots. Black spots are pigmented by melanin, whereas there was...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Pérez, M.M., Bochicchio, P.A., Rabossi, A., Quesada-Allué, L.A.
Formato: JOUR
Materias:
fly
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00221910_v107_n_p224_Perez
Aporte de:
id todo:paper_00221910_v107_n_p224_Perez
record_format dspace
spelling todo:paper_00221910_v107_n_p224_Perez2023-10-03T14:28:08Z Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings Pérez, M.M. Bochicchio, P.A. Rabossi, A. Quesada-Allué, L.A. Diptera N-beta-alanyldopamine N-beta-alanyldopamine-synthase Sclerotization Tanning Wings biological development cell color morph emergence enzyme enzyme activity fly melanin phenolic compound protein wing morphology Ceratitis capitata Diptera Drosophila melanogaster Punctum blandianum After the emergence of the Ceratitis capitata imago, the pale and folded wings are expanded and sclerotized to acquire the definitive form and to stabilize the cuticle. The wings of this fly show a specific pattern of brownish and black spots. Black spots are pigmented by melanin, whereas there was scarce information about the development of the brownish spots. N-beta-alanydopamine (NBAD) is the main tanning precursor in C. capitata body cuticle, and we hypothesized that it may be responsible for the colouration of the brownish spots. We determined the topology and timing of NBAD synthesis and deposition to attain the species-specific colouration pattern. We demonstrated that during the first hours the colour of the brownish spots was principally determined by the tanning of the hairs. Haemolymph circulation through the veins is required to tan the wings. We confirmed that soon after wing spreading, most of the wing epidermal cells disappeared. Thus, the tanning of the brown spots was accomplished when the wing lamina was devoid of cells. NBAD synthase (NBAD-S; Ebony protein in D. melanogaster) activity in wings was detected in pharate adults and lasted several days after the emergence, even after the end of the tanning process. This observation is in contrast to epidermal NBAD-S activity in the body, where it was nearly undetectable 48 h post emergence. Our results indicate that NBAD-S was exported and deposited into the extracellular matrix of the brown spot areas before cell death and that tanning occurs through gradual export of NBAD precursors (dopamine and b-alanine) from veins. © 2018 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221910_v107_n_p224_Perez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diptera
N-beta-alanyldopamine
N-beta-alanyldopamine-synthase
Sclerotization
Tanning
Wings
biological development
cell
color morph
emergence
enzyme
enzyme activity
fly
melanin
phenolic compound
protein
wing morphology
Ceratitis capitata
Diptera
Drosophila melanogaster
Punctum blandianum
spellingShingle Diptera
N-beta-alanyldopamine
N-beta-alanyldopamine-synthase
Sclerotization
Tanning
Wings
biological development
cell
color morph
emergence
enzyme
enzyme activity
fly
melanin
phenolic compound
protein
wing morphology
Ceratitis capitata
Diptera
Drosophila melanogaster
Punctum blandianum
Pérez, M.M.
Bochicchio, P.A.
Rabossi, A.
Quesada-Allué, L.A.
Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings
topic_facet Diptera
N-beta-alanyldopamine
N-beta-alanyldopamine-synthase
Sclerotization
Tanning
Wings
biological development
cell
color morph
emergence
enzyme
enzyme activity
fly
melanin
phenolic compound
protein
wing morphology
Ceratitis capitata
Diptera
Drosophila melanogaster
Punctum blandianum
description After the emergence of the Ceratitis capitata imago, the pale and folded wings are expanded and sclerotized to acquire the definitive form and to stabilize the cuticle. The wings of this fly show a specific pattern of brownish and black spots. Black spots are pigmented by melanin, whereas there was scarce information about the development of the brownish spots. N-beta-alanydopamine (NBAD) is the main tanning precursor in C. capitata body cuticle, and we hypothesized that it may be responsible for the colouration of the brownish spots. We determined the topology and timing of NBAD synthesis and deposition to attain the species-specific colouration pattern. We demonstrated that during the first hours the colour of the brownish spots was principally determined by the tanning of the hairs. Haemolymph circulation through the veins is required to tan the wings. We confirmed that soon after wing spreading, most of the wing epidermal cells disappeared. Thus, the tanning of the brown spots was accomplished when the wing lamina was devoid of cells. NBAD synthase (NBAD-S; Ebony protein in D. melanogaster) activity in wings was detected in pharate adults and lasted several days after the emergence, even after the end of the tanning process. This observation is in contrast to epidermal NBAD-S activity in the body, where it was nearly undetectable 48 h post emergence. Our results indicate that NBAD-S was exported and deposited into the extracellular matrix of the brown spot areas before cell death and that tanning occurs through gradual export of NBAD precursors (dopamine and b-alanine) from veins. © 2018 Elsevier Ltd
format JOUR
author Pérez, M.M.
Bochicchio, P.A.
Rabossi, A.
Quesada-Allué, L.A.
author_facet Pérez, M.M.
Bochicchio, P.A.
Rabossi, A.
Quesada-Allué, L.A.
author_sort Pérez, M.M.
title Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings
title_short Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings
title_full Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings
title_fullStr Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings
title_full_unstemmed Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings
title_sort extracellular activity of nbad-synthase is responsible for colouration of brown spots in ceratitis capitata wings
url http://hdl.handle.net/20.500.12110/paper_00221910_v107_n_p224_Perez
work_keys_str_mv AT perezmm extracellularactivityofnbadsynthaseisresponsibleforcolourationofbrownspotsinceratitiscapitatawings
AT bochicchiopa extracellularactivityofnbadsynthaseisresponsibleforcolourationofbrownspotsinceratitiscapitatawings
AT rabossia extracellularactivityofnbadsynthaseisresponsibleforcolourationofbrownspotsinceratitiscapitatawings
AT quesadaalluela extracellularactivityofnbadsynthaseisresponsibleforcolourationofbrownspotsinceratitiscapitatawings
_version_ 1807321466124894208