A Rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form β-(1→2) glucan
A mutant of Rhizobium meliloti that elicited the formation of inactive nodules in alfalfa was found not to form β-(1→2) glucan in vivo or in vitro. It was nonmotile because it lacks flagella. The 235-kilodalton protein which acts as an intermediate in β-(1→2) glucan synthesis was undetectable in the...
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| Autores principales: | , , , , , |
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| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219193_v169_n2_p880_Geremia |
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| Sumario: | A mutant of Rhizobium meliloti that elicited the formation of inactive nodules in alfalfa was found not to form β-(1→2) glucan in vivo or in vitro. It was nonmotile because it lacks flagella. The 235-kilodalton protein which acts as an intermediate in β-(1→2) glucan synthesis was undetectable in the mutant. These properties of the mutant are common to those of chvB mutants of Agrobacterium tumefaciens. Exopolysaccharide formation by the R. meliloti mutant was about double that by the wild type. |
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