Protein kinase A activity in permeabilized cells as an approximation to in vivo activity

Permeabilized germlings from the dimorphic fungus Mucor rouxii were used for in situ measurement of protein kinase A (PKA) activation, to compare the results with those obtained in vitro at low or high (nonlinear) enzyme concentrations. The apparent total activity per cell when measured in situ is 5...

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Detalles Bibliográficos
Autores principales:	Sorol, M.R., Pereyra, E., Mizyrycki, C., Rossi, S., Moreno, S.
Formato:	JOUR
Materias:	Activation cAMP cAMP analogs In situ permeabilization Mucor rouxii Nonlinear Protein kinase A cyclic AMP cyclic AMP dependent protein kinase holoenzyme polyamine sodium chloride animal cell article catalysis cell activity cell membrane permeability concentration response controlled study enzyme activation enzyme active site enzyme activity enzyme assay fungus growth measurement Mucor nonhuman priority journal Amylomyces rouxii Fungi
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00144827_v271_n2_p337_Sorol
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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