Differentiating agents modulate topoisomerase I activity in U-937 promonocytic cells

Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 3...

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Detalles Bibliográficos
Autores principales: Shayo, C.C., Mladovan, A.G., Baldi, A.
Formato: JOUR
Materias:
Differentiation
Topoisomerase I
U-937 cell
4alpha phorbol
dna topoisomerase
hexamethylenebisacetamide
phorbol 13 acetate 12 myristate
retinoic acid
article
cell differentiation
controlled study
enzyme activity
human
human cell
leukemia cell
priority journal
Acetamides
Antineoplastic Agents
Blotting, Northern
Blotting, Western
Carcinogens
Cell Transformation, Neoplastic
DNA Topoisomerases, Type I
Electrophoresis, Polyacrylamide Gel
Humans
Leukemia, Monocytic, Chronic
Tetradecanoylphorbol Acetate
Transcription, Genetic
Tretinoin
Tumor Cells, Cultured
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00142999_v324_n1_p129_Shayo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Phorbol 12-myristate 13-acetate (PMA), N,N'-hexamethylenebisacetamide (HMBA) and retinoic acid induce cell differentiation in U-937 promonocytic cells. This report examines the effects of these agents on DNA topoisomerase I activity. A decrease in enzyme activity could be detected as early as 30 min after treatment with all three differentiating compounds and lasted at least 48 h. No alteration in the levels of DNA topoisomerase I transcript or protein was observed during these treatments. The results might be explained by post-translational events that render DNA topoisomerase type I less active.

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