Binding of [3H]dexamethasone by rat placenta
[3H]Dexamethasone binding was measured in the basal zone trophoblast (BZT) and the labyrinthine zone (LZ) of placenta from Wistar rats on days 19–22 of pregnancy. Cytosol from both zones was incubated with [3H]dexamethasone for 4 h at 0–4 C, and high affinity, low capacity sites were measured by Sca...
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todo:paper_00137227_v108_n5_p1697_Heller2023-10-03T14:11:06Z Binding of [3H]dexamethasone by rat placenta Heller, C. Coirini, H. De Nicola, A.F. dexamethasone glucocorticoid receptor mercaptoethanol n ethylmaleimide steroid receptor tritium corticosterone dexamethasone drug receptor estradiol hormone receptor n ethylmaleimide progesterone promegestone radioisotope testosterone animal article binding competition cell nucleus cytosol female kinetics metabolism placenta pregnancy rat animal experiment corticosterone h 3 dexamethasone h 3 endocrine system female genital system placenta pregnancy promegestone h 3 Animal Binding, Competitive Cell Nucleus Cytosol Dexamethasone Ethylmaleimide Female Kinetics Mercaptoethanol Placenta Pregnancy Rats Receptors, Glucocorticoid Receptors, Steroid Support, Non-U.S. Gov't Tritium [3H]Dexamethasone binding was measured in the basal zone trophoblast (BZT) and the labyrinthine zone (LZ) of placenta from Wistar rats on days 19–22 of pregnancy. Cytosol from both zones was incubated with [3H]dexamethasone for 4 h at 0–4 C, and high affinity, low capacity sites were measured by Scatchard plot analysis in BZT and LZ, tissues which demonstrated similar concentrations of sites and affinity constants. Specific binding was decreased by omitting mercaptoethanol from the incubation buffer, by adding N-ethylmaleimide, or by increasing the temperature to 16 or 37 C. Competition studies revealed that the rank order of inhibition of [3H]dexamethasone binding by nonradioactive steroids was dexamethasone ≫ progesterone = corticosterone ≫ testosterone = 17α-estradiol. Dexamethasone-binding sites could be distinguished from progestinbinding sites by competition analysis as well as by the absence of 17α, 21-dimethyl-19-nor-pregna-4, 9-diene-3, 20-dione ([3H]-R5020) binding in LZ, whereas the corticoid was bound to both zones. Nuclei from BZT and LZ took up radioactive steroid when exposed to cytosol prelabeled with [3H]dexamethasone, but little radioactivity was present in nuclei incubated with buffer containing [3H]dexamethasone. After in vivo administration of [3H]dexamethasone to pregnant rats, radioactivity was recovered in nuclei and cytosol from both placental zones. Furthermore, whole homogenates or cytosol from BZT or LZ did not metabolize [3H]dexamethasone to any significant extent. These results suggest that rat placenta contains dexamethasonebinding sites which resemble the glucocorticoid receptors described in other tissues. © 1981 by The Endocrine Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v108_n5_p1697_Heller |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
dexamethasone glucocorticoid receptor mercaptoethanol n ethylmaleimide steroid receptor tritium corticosterone dexamethasone drug receptor estradiol hormone receptor n ethylmaleimide progesterone promegestone radioisotope testosterone animal article binding competition cell nucleus cytosol female kinetics metabolism placenta pregnancy rat animal experiment corticosterone h 3 dexamethasone h 3 endocrine system female genital system placenta pregnancy promegestone h 3 Animal Binding, Competitive Cell Nucleus Cytosol Dexamethasone Ethylmaleimide Female Kinetics Mercaptoethanol Placenta Pregnancy Rats Receptors, Glucocorticoid Receptors, Steroid Support, Non-U.S. Gov't Tritium |
| spellingShingle |
dexamethasone glucocorticoid receptor mercaptoethanol n ethylmaleimide steroid receptor tritium corticosterone dexamethasone drug receptor estradiol hormone receptor n ethylmaleimide progesterone promegestone radioisotope testosterone animal article binding competition cell nucleus cytosol female kinetics metabolism placenta pregnancy rat animal experiment corticosterone h 3 dexamethasone h 3 endocrine system female genital system placenta pregnancy promegestone h 3 Animal Binding, Competitive Cell Nucleus Cytosol Dexamethasone Ethylmaleimide Female Kinetics Mercaptoethanol Placenta Pregnancy Rats Receptors, Glucocorticoid Receptors, Steroid Support, Non-U.S. Gov't Tritium Heller, C. Coirini, H. De Nicola, A.F. Binding of [3H]dexamethasone by rat placenta |
| topic_facet |
dexamethasone glucocorticoid receptor mercaptoethanol n ethylmaleimide steroid receptor tritium corticosterone dexamethasone drug receptor estradiol hormone receptor n ethylmaleimide progesterone promegestone radioisotope testosterone animal article binding competition cell nucleus cytosol female kinetics metabolism placenta pregnancy rat animal experiment corticosterone h 3 dexamethasone h 3 endocrine system female genital system placenta pregnancy promegestone h 3 Animal Binding, Competitive Cell Nucleus Cytosol Dexamethasone Ethylmaleimide Female Kinetics Mercaptoethanol Placenta Pregnancy Rats Receptors, Glucocorticoid Receptors, Steroid Support, Non-U.S. Gov't Tritium |
| description |
[3H]Dexamethasone binding was measured in the basal zone trophoblast (BZT) and the labyrinthine zone (LZ) of placenta from Wistar rats on days 19–22 of pregnancy. Cytosol from both zones was incubated with [3H]dexamethasone for 4 h at 0–4 C, and high affinity, low capacity sites were measured by Scatchard plot analysis in BZT and LZ, tissues which demonstrated similar concentrations of sites and affinity constants. Specific binding was decreased by omitting mercaptoethanol from the incubation buffer, by adding N-ethylmaleimide, or by increasing the temperature to 16 or 37 C. Competition studies revealed that the rank order of inhibition of [3H]dexamethasone binding by nonradioactive steroids was dexamethasone ≫ progesterone = corticosterone ≫ testosterone = 17α-estradiol. Dexamethasone-binding sites could be distinguished from progestinbinding sites by competition analysis as well as by the absence of 17α, 21-dimethyl-19-nor-pregna-4, 9-diene-3, 20-dione ([3H]-R5020) binding in LZ, whereas the corticoid was bound to both zones. Nuclei from BZT and LZ took up radioactive steroid when exposed to cytosol prelabeled with [3H]dexamethasone, but little radioactivity was present in nuclei incubated with buffer containing [3H]dexamethasone. After in vivo administration of [3H]dexamethasone to pregnant rats, radioactivity was recovered in nuclei and cytosol from both placental zones. Furthermore, whole homogenates or cytosol from BZT or LZ did not metabolize [3H]dexamethasone to any significant extent. These results suggest that rat placenta contains dexamethasonebinding sites which resemble the glucocorticoid receptors described in other tissues. © 1981 by The Endocrine Society. |
| format |
JOUR |
| author |
Heller, C. Coirini, H. De Nicola, A.F. |
| author_facet |
Heller, C. Coirini, H. De Nicola, A.F. |
| author_sort |
Heller, C. |
| title |
Binding of [3H]dexamethasone by rat placenta |
| title_short |
Binding of [3H]dexamethasone by rat placenta |
| title_full |
Binding of [3H]dexamethasone by rat placenta |
| title_fullStr |
Binding of [3H]dexamethasone by rat placenta |
| title_full_unstemmed |
Binding of [3H]dexamethasone by rat placenta |
| title_sort |
binding of [3h]dexamethasone by rat placenta |
| url |
http://hdl.handle.net/20.500.12110/paper_00137227_v108_n5_p1697_Heller |
| work_keys_str_mv |
AT hellerc bindingof3hdexamethasonebyratplacenta AT coirinih bindingof3hdexamethasonebyratplacenta AT denicolaaf bindingof3hdexamethasonebyratplacenta |
| _version_ |
1807315382461005824 |