Glucosylation of Glycoproteins by Mammalian, Plant, Fungal, and Trypanosomatid Protozoa Microsomal Membranes

An assay for UDP-Glc:glycoprotein glucosyltransferase was developed. Incubation of rat liver microsomes with UDP-[14C]Glc led to the formation of hot trichloroacetic acid insoluble material identified as protein-linked Glc1Man7-9GlcNAc2. Addition of 8 M urea-denatured thyroglobulin to the incubation...

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Detalles Bibliográficos
Autores principales:	Trombetta, S.E., Bosch, M., Parodi, A.J.
Formato:	JOUR
Materias:	glycoprotein mannose radioisotope alpha glucosidase ab animal cell fungus glycogen(starch) synthase glycosylation liver microsome mammal microsome membrane nonhuman plant priority journal protozoon Acetylglucosaminidase Animal Crithidia Glucose Glucosyltransferases Glycoproteins Glycosylation Male Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Microsomes Microsomes, Liver Mucor Peptide Hydrolases Plants Protein Denaturation Rats Saccharomyces cerevisiae Streptomyces griseus Subcellular Fractions Support, Non-U.S. Gov't Transferases Trypanosoma cruzi
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00062960_v28_n20_p8108_Trombetta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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