Inhibition of polypeptide synthesis initiation by a change of Mg++ concentration in wheat germ cell-free systems
Polypeptide synthesis programmed by poly(U) and globin mRNA has been studied in cell-free extracts from wheat germ. A two-step reaction with a preincubation at high Mg++ levels followed by a second step carried out after a shift to a low Mg++ concentration and the addition of labeled amino acids is...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0006291x_v96_n1_p54_Algranati |
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| Sumario: | Polypeptide synthesis programmed by poly(U) and globin mRNA has been studied in cell-free extracts from wheat germ. A two-step reaction with a preincubation at high Mg++ levels followed by a second step carried out after a shift to a low Mg++ concentration and the addition of labeled amino acids is described. Under these conditions the initiation of polyphenylalanine synthesis can be blocked without affecting the elongation of polypeptide chains. This procedure allows the selective inhibition of polypeptide synthesis initiation without using any drug or antibiotic. © 1980, All rights reserved. |
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