Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2
Addition of methyl groups to arginine residues is catalyzed by a group of enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 is essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported to be expressed in nervous system and involved in neuro...
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todo:paper_0006291X_v473_n1_p194_Solari2023-10-03T14:04:04Z Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 Solari, C. Echegaray, C.V. Luzzani, C. Cosentino, M.S. Waisman, A. Petrone, M.V. Francia, M. Sassone, A. Canizo, J. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. Embryonic stem cells Induced pluripotent stem cells Nanog Oct4 Prmt8 Sox2 messenger RNA octamer transcription factor 4 protein arginine methyltransferase protein arginine methyltransferase 8 short hairpin RNA transcription factor NANOG transcription factor Sox2 unclassified drug homeodomain protein Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse PRMT8 protein, mouse protein arginine methyltransferase small interfering RNA Sox2 protein, mouse transcription factor Sox animal cell Article binding site cell differentiation controlled study down regulation embryo gene expression genetic transfection mouse mouse embryonic stem cell neural stem cell nonhuman nuclear reprogramming pluripotent stem cell priority journal promoter region upregulation 3T3 cell line animal cell differentiation cytology fibroblast gene expression regulation metabolism nerve cell pluripotent stem cell reverse transcription polymerase chain reaction Animals Cell Differentiation Down-Regulation Fibroblasts Gene Expression Regulation, Enzymologic Homeodomain Proteins Mice Neurons NIH 3T3 Cells Octamer Transcription Factor-3 Pluripotent Stem Cells Promoter Regions, Genetic Protein-Arginine N-Methyltransferases Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering SOXB1 Transcription Factors Transfection Addition of methyl groups to arginine residues is catalyzed by a group of enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 is essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported to be expressed in nervous system and involved in neurogenesis. In this work, we found that Prmt8 is expressed in mouse embryonic stem cells (ESC) and in induced pluripotent stem cells, and modulated along differentiation to neural precursor cells. We found that Prmt8 promoter activity is induced by the pluripotency transcription factors Oct4, Sox2 and Nanog. Moreover, endogenous Prmt8 mRNA levels were reduced in ESC transfected with Sox2 shRNA vector. As a whole, our results indicate that Prmt8 is expressed in pluripotent stem cells and its transcription is modulated by pluripotency transcription factors. These findings suggest that besides its known function in nervous system, Prmt8 could play a role in pluripotent stem cells. © 2016 Elsevier Inc. All rights reserved. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cosentino, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sassone, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v473_n1_p194_Solari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Embryonic stem cells Induced pluripotent stem cells Nanog Oct4 Prmt8 Sox2 messenger RNA octamer transcription factor 4 protein arginine methyltransferase protein arginine methyltransferase 8 short hairpin RNA transcription factor NANOG transcription factor Sox2 unclassified drug homeodomain protein Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse PRMT8 protein, mouse protein arginine methyltransferase small interfering RNA Sox2 protein, mouse transcription factor Sox animal cell Article binding site cell differentiation controlled study down regulation embryo gene expression genetic transfection mouse mouse embryonic stem cell neural stem cell nonhuman nuclear reprogramming pluripotent stem cell priority journal promoter region upregulation 3T3 cell line animal cell differentiation cytology fibroblast gene expression regulation metabolism nerve cell pluripotent stem cell reverse transcription polymerase chain reaction Animals Cell Differentiation Down-Regulation Fibroblasts Gene Expression Regulation, Enzymologic Homeodomain Proteins Mice Neurons NIH 3T3 Cells Octamer Transcription Factor-3 Pluripotent Stem Cells Promoter Regions, Genetic Protein-Arginine N-Methyltransferases Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering SOXB1 Transcription Factors Transfection |
| spellingShingle |
Embryonic stem cells Induced pluripotent stem cells Nanog Oct4 Prmt8 Sox2 messenger RNA octamer transcription factor 4 protein arginine methyltransferase protein arginine methyltransferase 8 short hairpin RNA transcription factor NANOG transcription factor Sox2 unclassified drug homeodomain protein Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse PRMT8 protein, mouse protein arginine methyltransferase small interfering RNA Sox2 protein, mouse transcription factor Sox animal cell Article binding site cell differentiation controlled study down regulation embryo gene expression genetic transfection mouse mouse embryonic stem cell neural stem cell nonhuman nuclear reprogramming pluripotent stem cell priority journal promoter region upregulation 3T3 cell line animal cell differentiation cytology fibroblast gene expression regulation metabolism nerve cell pluripotent stem cell reverse transcription polymerase chain reaction Animals Cell Differentiation Down-Regulation Fibroblasts Gene Expression Regulation, Enzymologic Homeodomain Proteins Mice Neurons NIH 3T3 Cells Octamer Transcription Factor-3 Pluripotent Stem Cells Promoter Regions, Genetic Protein-Arginine N-Methyltransferases Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering SOXB1 Transcription Factors Transfection Solari, C. Echegaray, C.V. Luzzani, C. Cosentino, M.S. Waisman, A. Petrone, M.V. Francia, M. Sassone, A. Canizo, J. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 |
| topic_facet |
Embryonic stem cells Induced pluripotent stem cells Nanog Oct4 Prmt8 Sox2 messenger RNA octamer transcription factor 4 protein arginine methyltransferase protein arginine methyltransferase 8 short hairpin RNA transcription factor NANOG transcription factor Sox2 unclassified drug homeodomain protein Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse PRMT8 protein, mouse protein arginine methyltransferase small interfering RNA Sox2 protein, mouse transcription factor Sox animal cell Article binding site cell differentiation controlled study down regulation embryo gene expression genetic transfection mouse mouse embryonic stem cell neural stem cell nonhuman nuclear reprogramming pluripotent stem cell priority journal promoter region upregulation 3T3 cell line animal cell differentiation cytology fibroblast gene expression regulation metabolism nerve cell pluripotent stem cell reverse transcription polymerase chain reaction Animals Cell Differentiation Down-Regulation Fibroblasts Gene Expression Regulation, Enzymologic Homeodomain Proteins Mice Neurons NIH 3T3 Cells Octamer Transcription Factor-3 Pluripotent Stem Cells Promoter Regions, Genetic Protein-Arginine N-Methyltransferases Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering SOXB1 Transcription Factors Transfection |
| description |
Addition of methyl groups to arginine residues is catalyzed by a group of enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 is essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported to be expressed in nervous system and involved in neurogenesis. In this work, we found that Prmt8 is expressed in mouse embryonic stem cells (ESC) and in induced pluripotent stem cells, and modulated along differentiation to neural precursor cells. We found that Prmt8 promoter activity is induced by the pluripotency transcription factors Oct4, Sox2 and Nanog. Moreover, endogenous Prmt8 mRNA levels were reduced in ESC transfected with Sox2 shRNA vector. As a whole, our results indicate that Prmt8 is expressed in pluripotent stem cells and its transcription is modulated by pluripotency transcription factors. These findings suggest that besides its known function in nervous system, Prmt8 could play a role in pluripotent stem cells. © 2016 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Solari, C. Echegaray, C.V. Luzzani, C. Cosentino, M.S. Waisman, A. Petrone, M.V. Francia, M. Sassone, A. Canizo, J. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. |
| author_facet |
Solari, C. Echegaray, C.V. Luzzani, C. Cosentino, M.S. Waisman, A. Petrone, M.V. Francia, M. Sassone, A. Canizo, J. Sevlever, G. Barañao, L. Miriuka, S. Guberman, A. |
| author_sort |
Solari, C. |
| title |
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 |
| title_short |
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 |
| title_full |
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 |
| title_fullStr |
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 |
| title_full_unstemmed |
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2 |
| title_sort |
protein arginine methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor sox2 |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v473_n1_p194_Solari |
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