Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium

Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing i...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Solari, C., Losino, N., Luzzani, C., Waisman, A., Bluguermann, C., Questa, M., Sevlever, G., Miriuka, S., Barañao, L., Guberman, A.
Formato: JOUR
Materias:
BGC
BGC-CM
CM
Conditioned medium
Culture conditions
ESC
GC
Induced pluripotent stem cell
IPSC
LIF
MEF
MESC
Pluripotency
Self-renewal
SM
animal cell
article
cell differentiation
cell type
controlled study
fibroblast
gene expression
granulosa cell
immunofluorescence
in vitro study
in vivo study
Lentivirinae
marker gene
mouse
nonhuman
pluripotent stem cell
priority journal
Animals
Cattle
Cell Culture Techniques
Cell Line
Culture Media, Conditioned
Female
Granulosa Cells
Induced Pluripotent Stem Cells
Mice
Octamer Transcription Factor-3
Regeneration
Bovinae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest.In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency. © 2011 Elsevier Inc.

Ejemplares similares