Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication

Leukaemia inhibitory factor (LIF) or Oncostatin M (OSM), both mitogens for Swiss mouse 3T3 cells, triggers initiation of DNA synthesis without the requirement for mevalonic acid. Thus, Lovastatin (LOV), an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM indu...

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Autores principales: Sauane, M., Coso, O.A., Giulianelli, S., Giráldez, A.N., Rudland, P.S., Jimenez De Asua, L.
Formato: JOUR
Materias:
DNA replication
Insulin
Leukaemia inhibitory factor
Mevalonic acid
Oncostatin M
3 hydroxy 3 methylglutaryl coenzyme A
DNA
leukemia inhibitory factor
mevalonic acid
mevinolin
oncostatin M
animal cell
article
cell cycle S phase
cell division
cell strain 3T3
controlled study
leukemia
nonhuman
priority journal
protein induction
protein processing
signal transduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v313_n4_p926_Sauane
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0006291X_v313_n4_p926_Sauane
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spelling todo:paper_0006291X_v313_n4_p926_Sauane2023-10-03T14:03:49Z Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication Sauane, M. Coso, O.A. Giulianelli, S. Giráldez, A.N. Rudland, P.S. Jimenez De Asua, L. DNA replication Insulin Leukaemia inhibitory factor Mevalonic acid Oncostatin M 3 hydroxy 3 methylglutaryl coenzyme A DNA leukemia inhibitory factor mevalonic acid mevinolin oncostatin M animal cell article cell cycle S phase cell division cell strain 3T3 controlled study DNA replication leukemia nonhuman priority journal protein induction protein processing signal transduction Leukaemia inhibitory factor (LIF) or Oncostatin M (OSM), both mitogens for Swiss mouse 3T3 cells, triggers initiation of DNA synthesis without the requirement for mevalonic acid. Thus, Lovastatin (LOV), an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM induced DNA replication and cell multiplication. In contrast, increasing concentrations of LOV from 1 to 60 μM block the mitogenic action of PGF 2α by decreasing the number of cells capable of entering S-phase and dividing. This inhibition by LOV can be reversed by addition of mevanolactone (MEV), an analogue of mevalonic acid. Thus, LIF or OSM triggers initiation of DNA replication independently of mevalonic acid synthesis and therefore without the involvement of isoprenylation of various signalling proteins. © 2003 Elsevier Inc. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v313_n4_p926_Sauane
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic DNA replication
Insulin
Leukaemia inhibitory factor
Mevalonic acid
Oncostatin M
3 hydroxy 3 methylglutaryl coenzyme A
DNA
leukemia inhibitory factor
mevalonic acid
mevinolin
oncostatin M
animal cell
article
cell cycle S phase
cell division
cell strain 3T3
controlled study
DNA replication
leukemia
nonhuman
priority journal
protein induction
protein processing
signal transduction
spellingShingle DNA replication
Insulin
Leukaemia inhibitory factor
Mevalonic acid
Oncostatin M
3 hydroxy 3 methylglutaryl coenzyme A
DNA
leukemia inhibitory factor
mevalonic acid
mevinolin
oncostatin M
animal cell
article
cell cycle S phase
cell division
cell strain 3T3
controlled study
DNA replication
leukemia
nonhuman
priority journal
protein induction
protein processing
signal transduction
Sauane, M.
Coso, O.A.
Giulianelli, S.
Giráldez, A.N.
Rudland, P.S.
Jimenez De Asua, L.
Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
topic_facet DNA replication
Insulin
Leukaemia inhibitory factor
Mevalonic acid
Oncostatin M
3 hydroxy 3 methylglutaryl coenzyme A
DNA
leukemia inhibitory factor
mevalonic acid
mevinolin
oncostatin M
animal cell
article
cell cycle S phase
cell division
cell strain 3T3
controlled study
DNA replication
leukemia
nonhuman
priority journal
protein induction
protein processing
signal transduction
description Leukaemia inhibitory factor (LIF) or Oncostatin M (OSM), both mitogens for Swiss mouse 3T3 cells, triggers initiation of DNA synthesis without the requirement for mevalonic acid. Thus, Lovastatin (LOV), an inhibitor of the hydroxy methylglutaryl CoA (HMGCoA) reductase, does not block LIF or OSM induced DNA replication and cell multiplication. In contrast, increasing concentrations of LOV from 1 to 60 μM block the mitogenic action of PGF 2α by decreasing the number of cells capable of entering S-phase and dividing. This inhibition by LOV can be reversed by addition of mevanolactone (MEV), an analogue of mevalonic acid. Thus, LIF or OSM triggers initiation of DNA replication independently of mevalonic acid synthesis and therefore without the involvement of isoprenylation of various signalling proteins. © 2003 Elsevier Inc. All rights reserved.
format JOUR
author Sauane, M.
Coso, O.A.
Giulianelli, S.
Giráldez, A.N.
Rudland, P.S.
Jimenez De Asua, L.
author_facet Sauane, M.
Coso, O.A.
Giulianelli, S.
Giráldez, A.N.
Rudland, P.S.
Jimenez De Asua, L.
author_sort Sauane, M.
title Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
title_short Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
title_full Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
title_fullStr Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
title_full_unstemmed Leukaemia inhibitory factor or Oncostatin M induction of Swiss 3T3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate DNA replication
title_sort leukaemia inhibitory factor or oncostatin m induction of swiss 3t3 cells does not require mevalonic acid synthesis nor protein isoprenylation to initiate dna replication
url http://hdl.handle.net/20.500.12110/paper_0006291X_v313_n4_p926_Sauane
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