Expression of a recombinant Fab antibody fragment against cruzipain, the major cysteine proteinase of Trypanosoma cruzi

Mostrar todas las versiones(2)

Cruzipain, the major proteinase of Trypanosoma cruzi, plays an important role in the biology of this parasite. This study reports the development of a recombinant Fab antibody, using RNA isolated from the anti-Ag163B6 hybridoma against cruzipain. This procedure involves the use of cDNAs obtained wit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kaplan, D., Baldi, C., Chiaramonte, M.G., Fernandez, M.M., Levin, M.J., Malchiodi, E., Baldi, A.
Formato:	JOUR
Materias:	complementary DNA cruzipain cysteine proteinase enzyme antibody immunoglobulin F(ab) fragment immunoglobulin heavy chain immunoglobulin kappa chain parasite antibody parasite antigen primer DNA recombinant antibody RNA animal cell antigen recognition article controlled study enzyme linked immunosorbent assay Escherichia coli gene expression system hybridoma immunofluorescence molecular cloning nonhuman nucleotide sequence priority journal Trypanosoma cruzi Animalia Trypanosoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v253_n1_p53_Kaplan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

Expression of a recombinant Fab antibody fragment against cruzipain, the major cysteine proteinase of Trypanosoma cruzi
Publicado: (1998)

Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi
por: Bontempi, Esteban José
Publicado: (1990)

Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi
por: Bontempi, E., et al.

Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
por: Pereira, C.M., et al.

Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein
Publicado: (1998)

Production and Characterization of Monoclonal Antibodies Against the Major Cysteine Proteinase of Trypanosoma cruzi
Publicado: (1994)

Production and Characterization of Monoclonal Antibodies Against the Major Cysteine Proteinase of Trypanosoma cruzi
por: GONZALEZ, G., et al.

Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
por: Malchiodi, E.L., et al.

Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
por: Martinez, Javier Augusto
Publicado: (1993)

Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins
por: López Bergami, P., et al.

Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease
por: Salinas, E., et al.

Presence of sialic acid in N-linked oligosaccharide chains and O-linked N-acetylglucosamine in cruzipain, the major cysteine proteinase of Trypanosoma cruzi
por: Barboza, M., et al.

Continuous-flow/stopped-flow system for enzyme immunoassay using a rotating bioreactor: Determination of Chagas disease
Publicado: (2005)

The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is antigenic in human infections
Publicado: (1991)

The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is antigenic in human infections
por: Martinez, J., et al.

Presence of sialic acid in N-linked oligosaccharide chains and O-linked N-acetylglucosamine in cruzipain, the major cysteine proteinase of Trypanosoma cruzi
por: Muchnik de Lederkremer, Rosa María, et al.
Publicado: (2003)

Reduced faecal shedding of Escherichia coli O157 H7 in cattle following systemic vaccination with Y - intimin C 280 and EspB proteins

Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins
Publicado: (2003)

The Trypanosoma cruzi ribosomal P protein family: Classification and antigenicity
por: Levin, M.J., et al.

Overexpression and refolding of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi: A component of the P1/P2/P0 complex
por: Juri Ayub, M., et al.

The Trypanosoma cruzi ribosomal P protein family: Classification and antigenicity
Publicado: (1993)

Overexpression and refolding of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi: A component of the P1/P2/P0 complex
Publicado: (2001)

The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons
por: Lorenzi, H.A., et al.

The VIPER elements of trypanosomes constitute a novel group of tyrosine recombinase-enconding retrotransposons
Publicado: (2006)

Trypanosoma cruzi adenylate cyclase activity. Purification and characterization
por: Torruella, M., et al.

Anomalous electrophoretic behaviour of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi in relation to its apparent molecular mass
por: Martinez, Javier Augusto
Publicado: (1992)

Anomalous electrophoretic behaviour of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi in relation to its apparent molecular mass
por: Martínez, J., et al.

DNA immunization with the ribosomal P2β gene of Trypanosoma cruzi fails to induce pathogenic antibodies
por: Levitus, G., et al.

Trypanosoma cruzi adenylate cyclase activity. Purification and characterization
por: Torruella, Mónica, et al.
Publicado: (1986)

Human recombinant antibodies against Trypanosoma cruzi ribosomal P2β protein
por: Grippo, V., et al.

DNA immunization with the ribosomal P2β gene of Trypanosoma cruzi fails to induce pathogenic antibodies
por: Levitus, Gabriela Laura, et al.
Publicado: (2003)

Systems biology approach to model the life cycle of Trypanosoma cruzi
por: Carrea, Alejandra, et al.
Publicado: (2016)

Human recombinant antibodies against Trypanosoma cruzi ribosomal P2β protein
Publicado: (2011)

Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily
por: Stoka, V., et al.

Chagas' disease: Humoral response to subcellular fraction of Trypanosoma cruzi in symptomatic and asymptomatic patients
por: De Titto, E.H., et al.

Inhibition of cruzipain, the major cysteine proteinase of the protozoan parasite, Trypanosoma cruzi, by proteinase inhibitors of the cystatin superfamily
por: Labriola, Carlos Alberto
Publicado: (1995)

Presence of sialic acid in N-linked oligosaccharide chains and O-linked N-acetylglucosamine in cruzipain, the major cysteine proteinase of Trypanosoma cruzi
por: Barboza, M., et al.

Chagas' disease: Humoral response to subcellular fraction of Trypanosoma cruzi in symptomatic and asymptomatic patients
Publicado: (1987)

The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression
por: Barderi, Patricia Alejandra, et al.
Publicado: (1998)

The NADP+ -linked glutamate dehydrogenase from Trypanosoma cruzi: Sequence, genomic organization and expression
por: Barderi, P., et al.