Mitoxantrone affects topoisomerase activities in human breast cancer cells
The effects of mitoxantrone, an antineoplastic DNA intercalator, on topoisomerase I and II were studied in two human breast cancer cell lines. A large increase of topoisomerase I activity was found when cells were exposed to various doses of mitoxantrone. Maximal effect was achieved with 20 and 40 n...
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todo:paper_0006291X_v136_n2_p521_Crespi2023-10-03T14:03:39Z Mitoxantrone affects topoisomerase activities in human breast cancer cells Crespi, M.D. Ivanier, S.E. Genovese, J. Baldi, A. dactinomycin dna dna topoisomerase mitoxantrone puromycin breast breast cancer cancer chemotherapy drug cytotoxicity enzyme inhibition human human cell intoxication priority journal therapy Anthraquinones Breast Neoplasms Cell Line Comparative Study Dactinomycin DNA Topoisomerases, Type I DNA Topoisomerases, Type II Doxorubicin Enzyme Induction Human Kinetics Mitoxantrone Puromycin Support, Non-U.S. Gov't The effects of mitoxantrone, an antineoplastic DNA intercalator, on topoisomerase I and II were studied in two human breast cancer cell lines. A large increase of topoisomerase I activity was found when cells were exposed to various doses of mitoxantrone. Maximal effect was achieved with 20 and 40 ng/mL in T47D and MCF-7 cells respectively. The enhancement on topoisomerase I activity seemed to be reversible, to be dependent on time of exposure to the drug and to require cellular integrity. Type II topoisomerase was inhibited in T47D cells after treatment for one hour with 10ng/mL of mitoxantrone and enzyme activity was undetectable at higher doses (40 ng/mL). This inhibitory effect did not take place in vitro unless the concentration of the intercalator was increased to 400-500 ng/mL. © 1986. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v136_n2_p521_Crespi |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
dactinomycin dna dna topoisomerase mitoxantrone puromycin breast breast cancer cancer chemotherapy drug cytotoxicity enzyme inhibition human human cell intoxication priority journal therapy Anthraquinones Breast Neoplasms Cell Line Comparative Study Dactinomycin DNA Topoisomerases, Type I DNA Topoisomerases, Type II Doxorubicin Enzyme Induction Human Kinetics Mitoxantrone Puromycin Support, Non-U.S. Gov't |
| spellingShingle |
dactinomycin dna dna topoisomerase mitoxantrone puromycin breast breast cancer cancer chemotherapy drug cytotoxicity enzyme inhibition human human cell intoxication priority journal therapy Anthraquinones Breast Neoplasms Cell Line Comparative Study Dactinomycin DNA Topoisomerases, Type I DNA Topoisomerases, Type II Doxorubicin Enzyme Induction Human Kinetics Mitoxantrone Puromycin Support, Non-U.S. Gov't Crespi, M.D. Ivanier, S.E. Genovese, J. Baldi, A. Mitoxantrone affects topoisomerase activities in human breast cancer cells |
| topic_facet |
dactinomycin dna dna topoisomerase mitoxantrone puromycin breast breast cancer cancer chemotherapy drug cytotoxicity enzyme inhibition human human cell intoxication priority journal therapy Anthraquinones Breast Neoplasms Cell Line Comparative Study Dactinomycin DNA Topoisomerases, Type I DNA Topoisomerases, Type II Doxorubicin Enzyme Induction Human Kinetics Mitoxantrone Puromycin Support, Non-U.S. Gov't |
| description |
The effects of mitoxantrone, an antineoplastic DNA intercalator, on topoisomerase I and II were studied in two human breast cancer cell lines. A large increase of topoisomerase I activity was found when cells were exposed to various doses of mitoxantrone. Maximal effect was achieved with 20 and 40 ng/mL in T47D and MCF-7 cells respectively. The enhancement on topoisomerase I activity seemed to be reversible, to be dependent on time of exposure to the drug and to require cellular integrity. Type II topoisomerase was inhibited in T47D cells after treatment for one hour with 10ng/mL of mitoxantrone and enzyme activity was undetectable at higher doses (40 ng/mL). This inhibitory effect did not take place in vitro unless the concentration of the intercalator was increased to 400-500 ng/mL. © 1986. |
| format |
JOUR |
| author |
Crespi, M.D. Ivanier, S.E. Genovese, J. Baldi, A. |
| author_facet |
Crespi, M.D. Ivanier, S.E. Genovese, J. Baldi, A. |
| author_sort |
Crespi, M.D. |
| title |
Mitoxantrone affects topoisomerase activities in human breast cancer cells |
| title_short |
Mitoxantrone affects topoisomerase activities in human breast cancer cells |
| title_full |
Mitoxantrone affects topoisomerase activities in human breast cancer cells |
| title_fullStr |
Mitoxantrone affects topoisomerase activities in human breast cancer cells |
| title_full_unstemmed |
Mitoxantrone affects topoisomerase activities in human breast cancer cells |
| title_sort |
mitoxantrone affects topoisomerase activities in human breast cancer cells |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v136_n2_p521_Crespi |
| work_keys_str_mv |
AT crespimd mitoxantroneaffectstopoisomeraseactivitiesinhumanbreastcancercells AT ivanierse mitoxantroneaffectstopoisomeraseactivitiesinhumanbreastcancercells AT genovesej mitoxantroneaffectstopoisomeraseactivitiesinhumanbreastcancercells AT baldia mitoxantroneaffectstopoisomeraseactivitiesinhumanbreastcancercells |
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1807319284449280000 |