Endotoxin detection in a competitive electrochemical assay: Synthesis of a suitable endotoxin conjugate

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A biotin-lipopolysaccharide (biotin-LPS) conjugate was synthesized from LPS smooth from Salmonella minnesota, yielding a conjugate with a biotin/LPS ratio equal to 1:1 and endotoxic activity of 0.08 EU ng-1. The conjugate was used in an amperometric competitive assay to determine endotoxins with end...

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Detalles Bibliográficos
Autores principales: Priano, G., Pallarola, D., Battaglini, F.
Formato: JOUR
Materias:
Biotin-conjugated lipopolysaccharide
Competitive assay
Lipopolysaccharide
biotin
carboxymethyldextran
cystamine
endotoxin
endotoxin neutralizing protein
gold
horseradish peroxidase
hydrogen peroxide
lipopolysaccharide
neutravidin
unclassified drug
amperometry
article
binding competition
chemical modification
concentration (parameters)
conjugation
covalent bond
electrochemical analysis
electrode
molecular recognition
nonhuman
oxidation reduction reaction
priority journal
Salmonella minnesota
toxin analysis
Biotin
Electrochemistry
Electrodes
Endotoxins
Lipopolysaccharides
Models, Biological
Models, Chemical
Molecular Structure
Oxidation-Reduction
Armoracia rusticana
Salmonella enterica subsp. enterica serovar Minnesota
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v362_n1_p108_Priano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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