Solid-phase assay for determination of binding parameters of ligand-protein complexes with high dissociation rates

The binding parameters, the affinity constant (K a ) and binding capacity (Q), of a protein possessing ligand-protein complexes with a high dissociation rate (Sex Steroid Binding protein from Bufo arenarum) were determined using a solid-phase method. The technique is based upon the adsorption of the...

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Detalles Bibliográficos
Autores principales: Santa-Coloma, T.A., Rossi, R.J., Charreau, E.H.
Formato: JOUR
Materias:
sex hormone binding protein
animal tissue
article
binding kinetics
dissociation
female
ligand binding
nonhuman
priority journal
protein binding
Animal
Bufo arenarum
Carrier Proteins
Charcoal
Chromatography, DEAE-Cellulose
Female
Half-Life
Kinetics
Ligands
Sex Hormone-Binding Globulin
Sex Hormones
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v192_n2_p367_SantaColoma
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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