Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype
We have used the nonpeptide angiotensin II (ANG II) receptor antagonists losartan (receptor subtype AT1) and PD-123319 (AT2) to determine the participation of ANG II receptor subtypes in luteinizing hormone-releasing hormone (LHRH)-induced prolactin release in a perifusion study using intact pituita...
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todo:paper_00029513_v266_n229-2_pE274_BecuVillalobos2023-10-03T13:54:57Z Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype Becu-Villalobos, D. Lacau-Mengido, I.M. Thyssen, S.M. Diaz-Torga, G.S. Libertun, C. angiotensin II receptor subtypes losartan luteinizing hormone-releasing hormone paracrine PD-123319 pituitary 1 (4 dimethylamino 3 methylbenzyl) 5 diphenylacetyl 4,5,6,7 tetrahydro 1h imidazo[4,5 c]pyridine 6 carboxylic acid angiotensin angiotensin receptor follitropin gonadorelin losartan losartan potassium luteinizing hormone prolactin receptor subtype adenohypophysis animal cell animal tissue article controlled study female follitropin release luteinizing hormone release nonhuman priority journal prolactin release prolactin secreting cell rat Angiotensin II Animal Biphenyl Compounds Female Gonadorelin Imidazoles In Vitro Losartan Perfusion Pituitary Gland Prolactin Pyridines Rats Rats, Sprague-Dawley Receptors, Angiotensin Support, Non-U.S. Gov't Tetrazoles We have used the nonpeptide angiotensin II (ANG II) receptor antagonists losartan (receptor subtype AT1) and PD-123319 (AT2) to determine the participation of ANG II receptor subtypes in luteinizing hormone-releasing hormone (LHRH)-induced prolactin release in a perifusion study using intact pituitaries in vitro. LHRH (1.85 x 10-7 M) released prolactin consistently, whereas losartan (10-5 M) abolished prolactin response without modifying basal prolactin or luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release. PD-123319 (10-5 M) had no effect on basal or LHRH-induced prolactin, LH, or FSH release. We also determined that the effect of ANG II on prolactin release was mediated by the same receptor subtype. In adenohypophysial cells dispersed in vitro ANG II (10-8 M) released prolactin. Losartan (10-7 and 10-6 M), but not PD-123319, inhibited this effect. We conclude that in intact hypophyses of 15-day-old female rats the effect of LHRH on prolactin release is readily demonstrated. LHRH-induced prolactin release appears to be mediated by ANG II acting in a paracrine manner on AT1 receptors located on lactotrophs. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00029513_v266_n229-2_pE274_BecuVillalobos |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
angiotensin II receptor subtypes losartan luteinizing hormone-releasing hormone paracrine PD-123319 pituitary 1 (4 dimethylamino 3 methylbenzyl) 5 diphenylacetyl 4,5,6,7 tetrahydro 1h imidazo[4,5 c]pyridine 6 carboxylic acid angiotensin angiotensin receptor follitropin gonadorelin losartan losartan potassium luteinizing hormone prolactin receptor subtype adenohypophysis animal cell animal tissue article controlled study female follitropin release luteinizing hormone release nonhuman priority journal prolactin release prolactin secreting cell rat Angiotensin II Animal Biphenyl Compounds Female Gonadorelin Imidazoles In Vitro Losartan Perfusion Pituitary Gland Prolactin Pyridines Rats Rats, Sprague-Dawley Receptors, Angiotensin Support, Non-U.S. Gov't Tetrazoles |
| spellingShingle |
angiotensin II receptor subtypes losartan luteinizing hormone-releasing hormone paracrine PD-123319 pituitary 1 (4 dimethylamino 3 methylbenzyl) 5 diphenylacetyl 4,5,6,7 tetrahydro 1h imidazo[4,5 c]pyridine 6 carboxylic acid angiotensin angiotensin receptor follitropin gonadorelin losartan losartan potassium luteinizing hormone prolactin receptor subtype adenohypophysis animal cell animal tissue article controlled study female follitropin release luteinizing hormone release nonhuman priority journal prolactin release prolactin secreting cell rat Angiotensin II Animal Biphenyl Compounds Female Gonadorelin Imidazoles In Vitro Losartan Perfusion Pituitary Gland Prolactin Pyridines Rats Rats, Sprague-Dawley Receptors, Angiotensin Support, Non-U.S. Gov't Tetrazoles Becu-Villalobos, D. Lacau-Mengido, I.M. Thyssen, S.M. Diaz-Torga, G.S. Libertun, C. Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype |
| topic_facet |
angiotensin II receptor subtypes losartan luteinizing hormone-releasing hormone paracrine PD-123319 pituitary 1 (4 dimethylamino 3 methylbenzyl) 5 diphenylacetyl 4,5,6,7 tetrahydro 1h imidazo[4,5 c]pyridine 6 carboxylic acid angiotensin angiotensin receptor follitropin gonadorelin losartan losartan potassium luteinizing hormone prolactin receptor subtype adenohypophysis animal cell animal tissue article controlled study female follitropin release luteinizing hormone release nonhuman priority journal prolactin release prolactin secreting cell rat Angiotensin II Animal Biphenyl Compounds Female Gonadorelin Imidazoles In Vitro Losartan Perfusion Pituitary Gland Prolactin Pyridines Rats Rats, Sprague-Dawley Receptors, Angiotensin Support, Non-U.S. Gov't Tetrazoles |
| description |
We have used the nonpeptide angiotensin II (ANG II) receptor antagonists losartan (receptor subtype AT1) and PD-123319 (AT2) to determine the participation of ANG II receptor subtypes in luteinizing hormone-releasing hormone (LHRH)-induced prolactin release in a perifusion study using intact pituitaries in vitro. LHRH (1.85 x 10-7 M) released prolactin consistently, whereas losartan (10-5 M) abolished prolactin response without modifying basal prolactin or luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release. PD-123319 (10-5 M) had no effect on basal or LHRH-induced prolactin, LH, or FSH release. We also determined that the effect of ANG II on prolactin release was mediated by the same receptor subtype. In adenohypophysial cells dispersed in vitro ANG II (10-8 M) released prolactin. Losartan (10-7 and 10-6 M), but not PD-123319, inhibited this effect. We conclude that in intact hypophyses of 15-day-old female rats the effect of LHRH on prolactin release is readily demonstrated. LHRH-induced prolactin release appears to be mediated by ANG II acting in a paracrine manner on AT1 receptors located on lactotrophs. |
| format |
JOUR |
| author |
Becu-Villalobos, D. Lacau-Mengido, I.M. Thyssen, S.M. Diaz-Torga, G.S. Libertun, C. |
| author_facet |
Becu-Villalobos, D. Lacau-Mengido, I.M. Thyssen, S.M. Diaz-Torga, G.S. Libertun, C. |
| author_sort |
Becu-Villalobos, D. |
| title |
Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype |
| title_short |
Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype |
| title_full |
Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype |
| title_fullStr |
Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype |
| title_full_unstemmed |
Effects of LHRH and ANG II on prolactin stimulation are mediated by hypophysial AT1 receptor subtype |
| title_sort |
effects of lhrh and ang ii on prolactin stimulation are mediated by hypophysial at1 receptor subtype |
| url |
http://hdl.handle.net/20.500.12110/paper_00029513_v266_n229-2_pE274_BecuVillalobos |
| work_keys_str_mv |
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1807321585573429248 |