GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration

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The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remai...

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Detalles Bibliográficos
Autores principales: Scolz, M., Widlund, P.O., Piazza, S., Bublik, D.R., Reber, S., Peche, L.Y., Ciani, Y., Hubner, N., Isokane, M., Monte, M., Ellenberg, J., Hyman, A.A., Schneider, C., Bird, A.W.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2012
Materias:
binding protein
end binding 1 protein
G2 and S phase expressed 1 protein
microtubule associated protein
microtubule plus end tracking protein
microtubule protein
unclassified drug
article
breast cancer
cancer cell culture
cell migration
controlled study
focal adhesion
human
human cell
microtubule
nucleotide sequence
protein function
protein protein interaction
regulatory mechanism
Breast Neoplasms
Cell Line
Cell Movement
DNA Primers
Female
Fluorescent Antibody Technique
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Immunoprecipitation
Kaplan-Meier Estimate
Mass Spectrometry
Microscopy, Fluorescence
Microtubule-Associated Proteins
Microtubules
Neoplasm Invasiveness
Real-Time Polymerase Chain Reaction
RNA Interference
RNA, Small Interfering
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19326203_v7_n12_p_Scolz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. © 2012 Scolz et al.

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