A quantitative real-time PCR method for in planta monitoring of Phytophthora infestans growth

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Aims: To establish a reliable and rapid protocol to simultaneously obtain high quality DNA from an infected host plant and the infecting pathogen. To develop an accurate and sensitive low-cost assay for the quantification and in planta monitoring of Phytophthora infestans growth.Methods and Results:...

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Detalles Bibliográficos
Autores principales: Llorente, B., Bravo-Almonacid, F., Cvitanich, C., Orlowska, E., Torres, H.N., Flawiá, M.M., Alonso, G.D.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2010
Materias:
Biomass monitoring
Late blight
Phytophthora infestans
Potato crop
Quantitative real-time PCR
Solanum tuberosum
adaptation
bioassay
cultivar
disease resistance
fungal disease
growth rate
host plant
host-pathogen interaction
monitoring system
plant breeding
polymerase chain reaction
real time
symptom
tuber
article
DNA purification
fungus growth
monitoring
nonhuman
nucleotide sequence
pathogenesis
plant
plant disease
plant gene
plant leaf
potato
quantitative analysis
real time polymerase chain reaction
DNA
DNA Primers
DNA, Plant
Organic Chemicals
Plant Diseases
Plant Leaves
Plant Tubers
Polymerase Chain Reaction
Species Specificity
Lycopersicon esculentum
Nicotiana benthamiana
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02668254_v51_n6_p603_Llorente
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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