Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms

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We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a...

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Detalles Bibliográficos
Autores principales: Fuxman Bass, J.I., Russo, D.M., Gabelloni, M.L., Geffner, J.R., Giordano, M., Catalano, M., Zorreguieta, Á., Trevani, A.S.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2010
Materias:
CD11b antigen
CD18 antigen
CD66b antigen
deoxyribonuclease I
DNA
interleukin 1beta
interleukin 8
lymphocyte antigen
unclassified drug
bacterial DNA
cytokine
article
bacterial strain
biofilm
controlled study
cytokine release
degradation
extracellular matrix
human
leukocyte activation
nonhuman
phagocytosis
priority journal
Pseudomonas aeruginosa
upregulation
biosynthesis
chemistry
confocal microscopy
extracellular fluid
growth, development and aging
immunology
metabolism
microbiology
neutrophil
physiology
Biofilms
Cytokines
DNA, Bacterial
Extracellular Fluid
Humans
Microscopy, Confocal
Neutrophil Activation
Neutrophils
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00221767_v184_n11_p6386_FuxmanBass
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a key component of biofilms of different bacterial species, the aim of this study was to determine whether it plays a role in the ability of biofilms to induce human neutrophil activation. We found that degradation of matrix extracellular DNA with DNase I markedly reduced the capacity of Pseudomonas aeruginosa biofilms to induce the release of the neutrophil proinflammatory cytokines IL-8 and IL-1β (>75%); reduced the upregulation of neutrophil activation markers CD18, CD11b, and CD66b (p < 0.001); reduced the number of bacteria phagocytosed per neutrophil contacting the biofilm; and reduced the production of neutrophil extracellular traps. Consistent with these findings, we found that biofilms formed by the lasI rhlI P. aeruginosa mutant strain, exhibiting a very low content of matrix extracellular DNA, displayed a lower capacity to stimulate the release of proinflammatory cytokines by neutrophils, which was not decreased further by DNase I treatment. Together, our findings support that matrix extracellular DNA is a major proinflammatory component of P. aeruginosa biofilms. Copyright © 2010 by The American Association of Immunologists, Inc.

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