Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors

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The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial p...

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Detalles Bibliográficos
Autores principales: Genovese, G., Senek, M., Ortiz, N., Regueira, M., Towle, D.W., Tresguerres, M., Luquet, C.M.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2006
Materias:
cAMP
Chasmagnathus granulatus
Crab
Dopamine antagonist
Dopamine receptor dopamine agonist
Na +/K+-ATPase
Okadaic acid
Transepithelial potential differences
adenosine triphosphatase (potassium sodium)
cyclic AMP dependent protein kinase
dopamine 1 receptor
dopamine 2 receptor
okadaic acid
theophylline
animal
article
Brachyura
gill
ion transport
male
metabolism
osmolarity
physiology
signal transduction
Animals
Cyclic AMP-Dependent Protein Kinases
Gills
Ion Transport
Male
Na(+)-K(+)-Exchanging ATPase
Okadaic Acid
Osmolar Concentration
Receptors, Dopamine D1
Receptors, Dopamine D2
Signal Transduction
Theophylline
Chasmagnathus granulata
Decapoda (Crustacea)
Hexapoda
Insecta
Vertebrata
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00220949_v209_n14_p2785_Genovese
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial potential difference (Vtc) from 2.2±0.2 to 4.8±0.3 mV, describing an initial cAMP-dependent stimulating phase followed by an inhibitory phase. Spiperone and domperidone (antagonists of D2-like DA receptors in vertebrates) completely blocked the response to DA, while the D1-like antagonist SCH23390 blocked only the inhibitory phase. Theophylline (phosphodiesterase inhibitor) and okadaic acid (protein phosphatases PP1 and PP2A inhibitor) were also able to block the inhibitory phase, suggesting that it depends on adenylyl cyclase inhibition and on protein phosphatases. When the gills were perfused with hypoosmotic solution, or with the adenylyl cyclase activator forskolin, Vte was increased several-fold. DA applied under these stimulated conditions partially reversed the Vte increase by 54% and 25%, respectively. Similarly, the D1-like agonist, fenoldopam, produced a 33% reduction in the stimulated Vte. We propose that, in C. granulatus gills, DA stimulates adenylyl cyclase and therefore ion transport through D1-like receptors linked to a Gs protein, although they respond to antagonists that interact with D2-like receptors in vertebrates. The inhibitory phase seems to be mediated by D2-like receptors linked to a Gi/o protein, which inhibits adenylyl cyclase, although these receptors can be activated or blocked by agonists or antagonists that interact with D1-like receptors in vertebrates and insects.

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