High affinity binding of melatonin in bovine medial basal hypothalamus
Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding s...
Guardado en:
| Publicado: |
1978
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_NIS18622_v6_n9_p357_Cardinali http://hdl.handle.net/20.500.12110/paper_NIS18622_v6_n9_p357_Cardinali |
| Aporte de: |
| Sumario: | Specific binding was linear up to 3 mg of protein in the incubation tube. Melatonin binding was pH-dependent the maximum being observed at pH 7.4. By adding 6 mM Ca++ to the buffer a 2-fold increase of specific binding was observed. Scatchard analysis revealed a population of high affinity binding sites with a Kd=(1.2±0.4)x10-8 M in 3 successive experiments. |
|---|