New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes

Sphingolipids are involved in a wide range of physiological and pathological processes none only as signaling molecules but also as key structural components regulating the lateral organization of cellular membranes. The preferential interaction of these biomolecules with cholesterol support the act...

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Autor principal: Levi, Valeria
Publicado: 2015
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816348_v_n_p1_Traian
http://hdl.handle.net/20.500.12110/paper_97816348_v_n_p1_Traian
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_97816348_v_n_p1_Traian2023-06-08T16:38:35Z New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes Levi, Valeria Sphingolipids are involved in a wide range of physiological and pathological processes none only as signaling molecules but also as key structural components regulating the lateral organization of cellular membranes. The preferential interaction of these biomolecules with cholesterol support the actual theory related with membrane heterogeneity in vivo, the raft theory. Rafts are believed to be highlydynamic and small domains enriched in sphingolipids, cholesterol and certain proteins present in the membrane of cells. The idea of these domains compartmentalizing cellular processes is a central hypothesis in biomedical research from immunology, virology, neurobiology to cancer. The use of microscopy to study lateral heterogeneity in biological membranes was developed during the nineties with the use of artificial models systems such as giant unillamelar vesicles and supported-lipid bilayers. The combination of confocal and two-photon microscopy techniques with fluorescent and solvatochromic probes like Laurdan enabled the acquisition of spatially-resolved information about the fluidity and/or order of artificial bilayers showing phase segregation. The development of new techniques combining Laurdan imaging with fluorescence fluctuation spectroscopy allowed the detection of highlypacked microdomains in natural cell membranes. In this article we review these exciting new approaches that open a window to further characterize these sphingolipid-enriched domains in cell membranes during both physiological and pathological processes. © 2015 by Nova Science Publishers, Inc. All rights reserved. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816348_v_n_p1_Traian http://hdl.handle.net/20.500.12110/paper_97816348_v_n_p1_Traian
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Sphingolipids are involved in a wide range of physiological and pathological processes none only as signaling molecules but also as key structural components regulating the lateral organization of cellular membranes. The preferential interaction of these biomolecules with cholesterol support the actual theory related with membrane heterogeneity in vivo, the raft theory. Rafts are believed to be highlydynamic and small domains enriched in sphingolipids, cholesterol and certain proteins present in the membrane of cells. The idea of these domains compartmentalizing cellular processes is a central hypothesis in biomedical research from immunology, virology, neurobiology to cancer. The use of microscopy to study lateral heterogeneity in biological membranes was developed during the nineties with the use of artificial models systems such as giant unillamelar vesicles and supported-lipid bilayers. The combination of confocal and two-photon microscopy techniques with fluorescent and solvatochromic probes like Laurdan enabled the acquisition of spatially-resolved information about the fluidity and/or order of artificial bilayers showing phase segregation. The development of new techniques combining Laurdan imaging with fluorescence fluctuation spectroscopy allowed the detection of highlypacked microdomains in natural cell membranes. In this article we review these exciting new approaches that open a window to further characterize these sphingolipid-enriched domains in cell membranes during both physiological and pathological processes. © 2015 by Nova Science Publishers, Inc. All rights reserved.
author Levi, Valeria
spellingShingle Levi, Valeria
New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
author_facet Levi, Valeria
author_sort Levi, Valeria
title New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
title_short New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
title_full New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
title_fullStr New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
title_full_unstemmed New fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: From artificial systems to cellular membranes
title_sort new fluorescence microscopy approaches to explore the influence of sphingolipids on lateral organization of biomembranes: from artificial systems to cellular membranes
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_97816348_v_n_p1_Traian
http://hdl.handle.net/20.500.12110/paper_97816348_v_n_p1_Traian
work_keys_str_mv AT levivaleria newfluorescencemicroscopyapproachestoexploretheinfluenceofsphingolipidsonlateralorganizationofbiomembranesfromartificialsystemstocellularmembranes
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