Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique
A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repre...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v16_n1_p59_Corton http://hdl.handle.net/20.500.12110/paper_87567938_v16_n1_p59_Corton |
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paper:paper_87567938_v16_n1_p59_Corton2023-06-08T16:36:54Z Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique Cortón, Eduardo Piuri, Mariana Battaglini, Fernando Ruzal, Sandra Mónica Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piuri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v16_n1_p59_Corton http://hdl.handle.net/20.500.12110/paper_87567938_v16_n1_p59_Corton |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus |
| spellingShingle |
Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus Cortón, Eduardo Piuri, Mariana Battaglini, Fernando Ruzal, Sandra Mónica Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| topic_facet |
Calcium alginate Catabolite Lactobacillus Lactose Monosaccharides bacterial mutant biomass bioreactor carbohydrate catabolite repression colony forming unit electrode fermentation immobilized biomass immobilized cell culture lactose pH quantitative analysis Bacteria Biomass Bioreactors Calcium compounds Carbohydrates Cell immobilization Glucose Growth kinetics Metabolism pH Reduction Fermentation Biomass Bioreactors Biotechnology Carbohydrate Metabolism Cells, Immobilized Fermentation Hydrogen-Ion Concentration Lactobacillus Lactobacillus casei Species Specificity Bacteria (microorganisms) Lactobacillus |
| description |
A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. A microbial bioreactor based on calcium alginate immobilized Lactobacillus cells coupled to a pH electrode was developed for quantitative determination of carbohydrate fermentation activity. A high biomass (1010 cfu mL-1) and particular pregrowth conditions were needed. Reduction of catabolite repression by monosaccharides was achieved by pregrowth in lactose. The evolution of acid production in a continuous flow-stopped flow bioreactor was monitored for different sugar solutions in contact with the immobilized bacteria. The resulting slopes (ΔmV/Δt) were used to quantify the fermentation capability for a defined sugar related to that of glucose, which was taken as 100%. The procedure is simple, being based on pH variation that can give quantitative results compared to other reported techniques for carbohydrate fermentation pattern from which only qualitative results are obtained. In addition, it offers reduction in time and costs and is a suitable tool for the rapid analysis of isolated strains and in studies of modifications of sugar metabolism in mutants. |
| author |
Cortón, Eduardo Piuri, Mariana Battaglini, Fernando Ruzal, Sandra Mónica |
| author_facet |
Cortón, Eduardo Piuri, Mariana Battaglini, Fernando Ruzal, Sandra Mónica |
| author_sort |
Cortón, Eduardo |
| title |
Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| title_short |
Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| title_full |
Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| title_fullStr |
Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| title_full_unstemmed |
Characterization of Lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| title_sort |
characterization of lactobacillus carbohydrate fermentation activity using immobilized cell technique |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_87567938_v16_n1_p59_Corton http://hdl.handle.net/20.500.12110/paper_87567938_v16_n1_p59_Corton |
| work_keys_str_mv |
AT cortoneduardo characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique AT piurimariana characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique AT battaglinifernando characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique AT ruzalsandramonica characterizationoflactobacilluscarbohydratefermentationactivityusingimmobilizedcelltechnique |
| _version_ |
1768543395500785664 |