Susceptibility of placental mitochondria to oxidative stress

Background: Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification repre...

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Publicado:	2018
Materias:	mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation protein carbonylation protein determination spectrophotometry
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24721727_v110_n16_p1228_PapaGobbi http://hdl.handle.net/20.500.12110/paper_24721727_v110_n16_p1228_PapaGobbi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_24721727_v110_n16_p1228_PapaGobbi
record_format	dspace
spelling	paper:paper_24721727_v110_n16_p1228_PapaGobbi2023-06-08T16:36:48Z Susceptibility of placental mitochondria to oxidative stress mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry Background: Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a serious threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and susceptibility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in placental HMF and LMF performed in a cell-free assay. The small number of antioxidant system parameters measured did not allow detecting differences between HMF and LMF. © 2018 Wiley Periodicals, Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24721727_v110_n16_p1228_PapaGobbi http://hdl.handle.net/20.500.12110/paper_24721727_v110_n16_p1228_PapaGobbi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry
spellingShingle	mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry Susceptibility of placental mitochondria to oxidative stress
topic_facet	mitochondria oxidative stress placenta reactive oxygen species catalase glutathione hydrogen peroxide malonaldehyde adult antioxidant activity Article cell isolation controlled study enzymatic assay enzyme activity female human human tissue lipid peroxidation mitochondrion normal human oxidation oxidative stress placenta protein carbonylation protein determination spectrophotometry
description	Background: Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a serious threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and susceptibility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in placental HMF and LMF performed in a cell-free assay. The small number of antioxidant system parameters measured did not allow detecting differences between HMF and LMF. © 2018 Wiley Periodicals, Inc.
title	Susceptibility of placental mitochondria to oxidative stress
title_short	Susceptibility of placental mitochondria to oxidative stress
title_full	Susceptibility of placental mitochondria to oxidative stress
title_fullStr	Susceptibility of placental mitochondria to oxidative stress
title_full_unstemmed	Susceptibility of placental mitochondria to oxidative stress
title_sort	susceptibility of placental mitochondria to oxidative stress
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_24721727_v110_n16_p1228_PapaGobbi http://hdl.handle.net/20.500.12110/paper_24721727_v110_n16_p1228_PapaGobbi
_version_	1768545305427443712

Susceptibility of placental mitochondria to oxidative stress

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