CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells

Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR...

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Autores principales:	Bonfiglio, Juan José, Silberstein Cuña, Susana Iris
Publicado:	2017
Materias:	adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v7_n1_p_Inda http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Inda
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_20452322_v7_n1_p_Inda2023-06-08T16:33:30Z CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells Bonfiglio, Juan José Silberstein Cuña, Susana Iris adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes. © 2017 The Author(s). Fil:Bonfiglio, J.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v7_n1_p_Inda http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Inda
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone
spellingShingle	adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone Bonfiglio, Juan José Silberstein Cuña, Susana Iris CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
topic_facet	adenylate cyclase biological marker corticotropin releasing factor corticotropin releasing factor receptor corticotropin releasing factor receptor 1 cyclic AMP cyclic AMP responsive element binding protein binding protein animal blood cell culture cell cycle checkpoint cell differentiation cell survival cytology human metabolism mouse pyramidal nerve cell Adenylyl Cyclases Animals Biomarkers Cell Cycle Checkpoints Cell Differentiation Cell Survival Cells, Cultured Corticotropin-Releasing Hormone CREB-Binding Protein Cyclic AMP Humans Mice Pyramidal Cells Receptors, Corticotropin-Releasing Hormone
description	Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes. © 2017 The Author(s).
author	Bonfiglio, Juan José Silberstein Cuña, Susana Iris
author_facet	Bonfiglio, Juan José Silberstein Cuña, Susana Iris
author_sort	Bonfiglio, Juan José
title	CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_short	CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_full	CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_fullStr	CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_full_unstemmed	CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
title_sort	camp-dependent cell differentiation triggered by activated crhr1 in hippocampal neuronal cells
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v7_n1_p_Inda http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Inda
work_keys_str_mv	AT bonfigliojuanjose campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells AT silbersteincunasusanairis campdependentcelldifferentiationtriggeredbyactivatedcrhr1inhippocampalneuronalcells
_version_	1768542811526791168

CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells

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