New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition

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Background: Human papillomavirus (HPV) is the main causative agent of cervical cancer, particularly high risk strains such us HPV-16, -18 and -31. The viral encoded E2 protein acts as a transcriptional modulator and exerts a key role in viral DNA replication. Thus, E2 constitutes an attractive targe...

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Autores principales:	Wetzler, Diana E., Comin, María Julieta, Gallo, Mariana
Publicado:	2011
Materias:	distamycin A DNA peptide protein E2 transcription factor unclassified drug antivirus agent biomimetic material DNA binding protein E2 protein, Human papillomavirus type 16 oncoprotein peptide fragment pyrrole derivative virus DNA alpha helix article circular dichroism computer model conjugation DNA synthesis gel mobility shift assay Human papillomavirus type 16 nonhuman nuclear magnetic resonance spectroscopy protein DNA binding protein interaction protein structure amino acid sequence chemical structure chemistry enzyme specificity genetics metabolism molecular genetics nucleotide sequence papillomavirus infection physiology protein secondary structure synthesis Human papillomavirus Amino Acid Sequence Antiviral Agents Base Sequence Biomimetic Materials Distamycins DNA, Viral DNA-Binding Proteins Human papillomavirus 16 Models, Molecular Molecular Sequence Data Oncogene Proteins, Viral Papillomavirus Infections Peptide Fragments Protein Structure, Secondary Pyrroles Substrate Specificity
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n7_p_Wetzler http://hdl.handle.net/20.500.12110/paper_19326203_v6_n7_p_Wetzler
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_19326203_v6_n7_p_Wetzler
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spelling	paper:paper_19326203_v6_n7_p_Wetzler2023-06-08T16:30:57Z New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition Wetzler, Diana E. Comin, María Julieta Gallo, Mariana distamycin A DNA peptide protein E2 transcription factor unclassified drug antivirus agent biomimetic material distamycin A DNA binding protein E2 protein, Human papillomavirus type 16 oncoprotein peptide fragment pyrrole derivative virus DNA alpha helix article circular dichroism computer model conjugation DNA synthesis gel mobility shift assay Human papillomavirus type 16 nonhuman nuclear magnetic resonance spectroscopy protein DNA binding protein interaction protein structure amino acid sequence chemical structure chemistry enzyme specificity genetics Human papillomavirus type 16 metabolism molecular genetics nucleotide sequence papillomavirus infection physiology protein secondary structure synthesis Human papillomavirus Human papillomavirus type 16 Amino Acid Sequence Antiviral Agents Base Sequence Biomimetic Materials Distamycins DNA, Viral DNA-Binding Proteins Human papillomavirus 16 Models, Molecular Molecular Sequence Data Oncogene Proteins, Viral Papillomavirus Infections Peptide Fragments Protein Structure, Secondary Pyrroles Substrate Specificity Background: Human papillomavirus (HPV) is the main causative agent of cervical cancer, particularly high risk strains such us HPV-16, -18 and -31. The viral encoded E2 protein acts as a transcriptional modulator and exerts a key role in viral DNA replication. Thus, E2 constitutes an attractive target for developing antiviral agents. E2 is a homodimeric protein that interacts with the DNA target through an α-helix of each monomer. However, a peptide corresponding to the DNA recognition helix of HPV-16 E2 binds DNA with lower affinity than its full-length DNA binding domain. Therefore, in an attempt to promote the DNA binding of the isolated peptide, we have designed a conjugate compound of the E2 α-helix peptide and a derivative of the antibiotic distamycin, which involves simultaneous minor- and major-groove interactions. Methodology/Principal Findings: An E2 α-helix peptide-distamycin conjugate was designed and synthesized. It was characterized by NMR and CD spectroscopy, and its DNA binding properties were investigated by CD, DNA melting and gel shift experiments. The coupling of E2 peptide with distamycin does not affect its structural properties. The conjugate improves significantly the affinity of the peptide for specific DNA. In addition, stoichiometric amounts of specific DNA increase meaningfully the helical population of the peptide. The conjugate enhances the DNA binding constant 50-fold, maintaining its specificity. Conclusions/Significance: These results demonstrate that peptide-distamycin conjugates are a promising tool to obtain compounds that bind the E2 target DNA-sequences with remarkable affinity and suggest that a bipartite major/minor groove binding scaffold can be a useful approach for therapeutic treatment of HPV infection. © 2011 Wetzler et al. Fil:Wetzler, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Comin, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gallo, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n7_p_Wetzler http://hdl.handle.net/20.500.12110/paper_19326203_v6_n7_p_Wetzler
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	distamycin A DNA peptide protein E2 transcription factor unclassified drug antivirus agent biomimetic material distamycin A DNA binding protein E2 protein, Human papillomavirus type 16 oncoprotein peptide fragment pyrrole derivative virus DNA alpha helix article circular dichroism computer model conjugation DNA synthesis gel mobility shift assay Human papillomavirus type 16 nonhuman nuclear magnetic resonance spectroscopy protein DNA binding protein interaction protein structure amino acid sequence chemical structure chemistry enzyme specificity genetics Human papillomavirus type 16 metabolism molecular genetics nucleotide sequence papillomavirus infection physiology protein secondary structure synthesis Human papillomavirus Human papillomavirus type 16 Amino Acid Sequence Antiviral Agents Base Sequence Biomimetic Materials Distamycins DNA, Viral DNA-Binding Proteins Human papillomavirus 16 Models, Molecular Molecular Sequence Data Oncogene Proteins, Viral Papillomavirus Infections Peptide Fragments Protein Structure, Secondary Pyrroles Substrate Specificity
spellingShingle	distamycin A DNA peptide protein E2 transcription factor unclassified drug antivirus agent biomimetic material distamycin A DNA binding protein E2 protein, Human papillomavirus type 16 oncoprotein peptide fragment pyrrole derivative virus DNA alpha helix article circular dichroism computer model conjugation DNA synthesis gel mobility shift assay Human papillomavirus type 16 nonhuman nuclear magnetic resonance spectroscopy protein DNA binding protein interaction protein structure amino acid sequence chemical structure chemistry enzyme specificity genetics Human papillomavirus type 16 metabolism molecular genetics nucleotide sequence papillomavirus infection physiology protein secondary structure synthesis Human papillomavirus Human papillomavirus type 16 Amino Acid Sequence Antiviral Agents Base Sequence Biomimetic Materials Distamycins DNA, Viral DNA-Binding Proteins Human papillomavirus 16 Models, Molecular Molecular Sequence Data Oncogene Proteins, Viral Papillomavirus Infections Peptide Fragments Protein Structure, Secondary Pyrroles Substrate Specificity Wetzler, Diana E. Comin, María Julieta Gallo, Mariana New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
topic_facet	distamycin A DNA peptide protein E2 transcription factor unclassified drug antivirus agent biomimetic material distamycin A DNA binding protein E2 protein, Human papillomavirus type 16 oncoprotein peptide fragment pyrrole derivative virus DNA alpha helix article circular dichroism computer model conjugation DNA synthesis gel mobility shift assay Human papillomavirus type 16 nonhuman nuclear magnetic resonance spectroscopy protein DNA binding protein interaction protein structure amino acid sequence chemical structure chemistry enzyme specificity genetics Human papillomavirus type 16 metabolism molecular genetics nucleotide sequence papillomavirus infection physiology protein secondary structure synthesis Human papillomavirus Human papillomavirus type 16 Amino Acid Sequence Antiviral Agents Base Sequence Biomimetic Materials Distamycins DNA, Viral DNA-Binding Proteins Human papillomavirus 16 Models, Molecular Molecular Sequence Data Oncogene Proteins, Viral Papillomavirus Infections Peptide Fragments Protein Structure, Secondary Pyrroles Substrate Specificity
description	Background: Human papillomavirus (HPV) is the main causative agent of cervical cancer, particularly high risk strains such us HPV-16, -18 and -31. The viral encoded E2 protein acts as a transcriptional modulator and exerts a key role in viral DNA replication. Thus, E2 constitutes an attractive target for developing antiviral agents. E2 is a homodimeric protein that interacts with the DNA target through an α-helix of each monomer. However, a peptide corresponding to the DNA recognition helix of HPV-16 E2 binds DNA with lower affinity than its full-length DNA binding domain. Therefore, in an attempt to promote the DNA binding of the isolated peptide, we have designed a conjugate compound of the E2 α-helix peptide and a derivative of the antibiotic distamycin, which involves simultaneous minor- and major-groove interactions. Methodology/Principal Findings: An E2 α-helix peptide-distamycin conjugate was designed and synthesized. It was characterized by NMR and CD spectroscopy, and its DNA binding properties were investigated by CD, DNA melting and gel shift experiments. The coupling of E2 peptide with distamycin does not affect its structural properties. The conjugate improves significantly the affinity of the peptide for specific DNA. In addition, stoichiometric amounts of specific DNA increase meaningfully the helical population of the peptide. The conjugate enhances the DNA binding constant 50-fold, maintaining its specificity. Conclusions/Significance: These results demonstrate that peptide-distamycin conjugates are a promising tool to obtain compounds that bind the E2 target DNA-sequences with remarkable affinity and suggest that a bipartite major/minor groove binding scaffold can be a useful approach for therapeutic treatment of HPV infection. © 2011 Wetzler et al.
author	Wetzler, Diana E. Comin, María Julieta Gallo, Mariana
author_facet	Wetzler, Diana E. Comin, María Julieta Gallo, Mariana
author_sort	Wetzler, Diana E.
title	New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
title_short	New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
title_full	New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
title_fullStr	New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
title_full_unstemmed	New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
title_sort	new human papilloma virus e2 transcription factor mimics: a tripyrrole-peptide conjugate with tight and specific dna-recognition
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n7_p_Wetzler http://hdl.handle.net/20.500.12110/paper_19326203_v6_n7_p_Wetzler
work_keys_str_mv	AT wetzlerdianae newhumanpapillomaviruse2transcriptionfactormimicsatripyrrolepeptideconjugatewithtightandspecificdnarecognition AT cominmariajulieta newhumanpapillomaviruse2transcriptionfactormimicsatripyrrolepeptideconjugatewithtightandspecificdnarecognition AT gallomariana newhumanpapillomaviruse2transcriptionfactormimicsatripyrrolepeptideconjugatewithtightandspecificdnarecognition
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New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition

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