Association between mast cells of different phenotypes and angiogenesis in colorectal cancer
It is known that mast cells proliferate in solid tumours and increase tumour angiogenesis. Nevertheless, there is no consensus regarding their role in colorectal cancer (CRC). In this study, we aimed to clarify the relationship of mast cells positive for tryptase (MCts) and tryptase-chymase (MCtcs)...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17912997_v1_n6_p895_Mauro http://hdl.handle.net/20.500.12110/paper_17912997_v1_n6_p895_Mauro |
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paper:paper_17912997_v1_n6_p895_Mauro2023-06-08T16:29:06Z Association between mast cells of different phenotypes and angiogenesis in colorectal cancer Mauro, Laura Valeria Puricelli, Lydia Inés Angiogenesis Colorectal carcinoma Immunohistochemistry Mast cell chymase Mast cell tryptase chymase tryptase angiogenesis article cancer staging cancer survival cell proliferation colorectal cancer controlled study correlation analysis disease association enzyme activity human human cell human tissue immunohistochemistry mast cell microscopy overall survival phenotype retrospective study survival rate It is known that mast cells proliferate in solid tumours and increase tumour angiogenesis. Nevertheless, there is no consensus regarding their role in colorectal cancer (CRC). In this study, we aimed to clarify the relationship of mast cells positive for tryptase (MCts) and tryptase-chymase (MCtcs) with microvessel density (MVD) in the intratumoral zone and the invasive edge of 80 CRC patient tumours. We evaluated these parameters and associated their expression with clinicopathological parameters, including survival rate. Tumour sections from each patient were immunostained for tryptase to evaluate MCts, chymase to evaluate MCtcs, and CD34 to evaluate microvessel counts under x100 microscopy. The number of MCs of both phenotypes and the MVD counts were higher in the invasive edge than in the intratumoral zone (p<0.001). MCt numbers were higher than those of MCtcs in all Astler-Coller stages in both regions. A positive correlation between MVD and MCts or MCtcs was observed (Pearson's test p<0.001). Neither the number of MCs nor MVD was associated with overall survival (log rank test). However, only 8.3% of patients with low numbers of MCtcs in the invasive edge succumbed to the disease, compared to 32% with high numbers of MCtcs. Our results indicate that angiogenesis and MC hyperplasia are events which appear early during CRC development. The correlation of MC phenotypes with MVD is in agreement with the role attributed to MCs, that of angiogenesis enhancement. Collectively, these findings suggest that screening during the early malignization of CRC can provide valuable clinical information. Fil:Mauro, L.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Puricelli, L.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17912997_v1_n6_p895_Mauro http://hdl.handle.net/20.500.12110/paper_17912997_v1_n6_p895_Mauro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Angiogenesis Colorectal carcinoma Immunohistochemistry Mast cell chymase Mast cell tryptase chymase tryptase angiogenesis article cancer staging cancer survival cell proliferation colorectal cancer controlled study correlation analysis disease association enzyme activity human human cell human tissue immunohistochemistry mast cell microscopy overall survival phenotype retrospective study survival rate |
| spellingShingle |
Angiogenesis Colorectal carcinoma Immunohistochemistry Mast cell chymase Mast cell tryptase chymase tryptase angiogenesis article cancer staging cancer survival cell proliferation colorectal cancer controlled study correlation analysis disease association enzyme activity human human cell human tissue immunohistochemistry mast cell microscopy overall survival phenotype retrospective study survival rate Mauro, Laura Valeria Puricelli, Lydia Inés Association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| topic_facet |
Angiogenesis Colorectal carcinoma Immunohistochemistry Mast cell chymase Mast cell tryptase chymase tryptase angiogenesis article cancer staging cancer survival cell proliferation colorectal cancer controlled study correlation analysis disease association enzyme activity human human cell human tissue immunohistochemistry mast cell microscopy overall survival phenotype retrospective study survival rate |
| description |
It is known that mast cells proliferate in solid tumours and increase tumour angiogenesis. Nevertheless, there is no consensus regarding their role in colorectal cancer (CRC). In this study, we aimed to clarify the relationship of mast cells positive for tryptase (MCts) and tryptase-chymase (MCtcs) with microvessel density (MVD) in the intratumoral zone and the invasive edge of 80 CRC patient tumours. We evaluated these parameters and associated their expression with clinicopathological parameters, including survival rate. Tumour sections from each patient were immunostained for tryptase to evaluate MCts, chymase to evaluate MCtcs, and CD34 to evaluate microvessel counts under x100 microscopy. The number of MCs of both phenotypes and the MVD counts were higher in the invasive edge than in the intratumoral zone (p<0.001). MCt numbers were higher than those of MCtcs in all Astler-Coller stages in both regions. A positive correlation between MVD and MCts or MCtcs was observed (Pearson's test p<0.001). Neither the number of MCs nor MVD was associated with overall survival (log rank test). However, only 8.3% of patients with low numbers of MCtcs in the invasive edge succumbed to the disease, compared to 32% with high numbers of MCtcs. Our results indicate that angiogenesis and MC hyperplasia are events which appear early during CRC development. The correlation of MC phenotypes with MVD is in agreement with the role attributed to MCs, that of angiogenesis enhancement. Collectively, these findings suggest that screening during the early malignization of CRC can provide valuable clinical information. |
| author |
Mauro, Laura Valeria Puricelli, Lydia Inés |
| author_facet |
Mauro, Laura Valeria Puricelli, Lydia Inés |
| author_sort |
Mauro, Laura Valeria |
| title |
Association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| title_short |
Association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| title_full |
Association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| title_fullStr |
Association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| title_full_unstemmed |
Association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| title_sort |
association between mast cells of different phenotypes and angiogenesis in colorectal cancer |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17912997_v1_n6_p895_Mauro http://hdl.handle.net/20.500.12110/paper_17912997_v1_n6_p895_Mauro |
| work_keys_str_mv |
AT maurolauravaleria associationbetweenmastcellsofdifferentphenotypesandangiogenesisincolorectalcancer AT puricellilydiaines associationbetweenmastcellsofdifferentphenotypesandangiogenesisincolorectalcancer |
| _version_ |
1768543677844553728 |