A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement

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Introduction: Mesenchymal stem cells (MSCs) are a promising source of cells for regenerative therapies. Although they can be isolated easily from several tissues, cell expansion is limited since their properties are lost with successive passages. Hence, pluripotent derived MSCs (PD-MSCs) arise as a...

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Autores principales:	Luzzani, Carlos Daniel, Questa, María, Guberman, Alejandra Sonia, Scassa, María Elida, Romorini, Leonardo
Publicado:	2015
Materias:	5' nucleotidase activated leukocyte cell adhesion molecule CD271 antigen cell surface marker endoglin integrin lymphocyte antigen octamer transcription factor 4 scleroprotein Thy 1 antigen unclassified drug membrane antigen signal peptide antigen expression Article cell differentiation cell lineage cell lysate cell stimulation controlled study culture medium DNA methylation embryo flow cytometry human human cell immunomodulation lymphocyte mesenchymal stem cell peripheral blood mononuclear cell platelet lysate pluripotent stem cell priority journal promoter region protein analysis protein expression stem cell expansion umbilical cord cell culture cytology drug effects fluorescence microscopy human embryonic stem cell mesenchymal stroma cell metabolism phenotype thrombocyte Bovinae Antigens, Surface Blood Platelets Cell Differentiation Cells, Cultured DNA Methylation Human Embryonic Stem Cells Humans Intercellular Signaling Peptides and Proteins Mesenchymal Stromal Cells Microscopy, Fluorescence Phenotype Pluripotent Stem Cells Promoter Regions, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17576512_v6_n1_p_Luzzani http://hdl.handle.net/20.500.12110/paper_17576512_v6_n1_p_Luzzani
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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