DNAzymes and ribozymes carrying 2'-C-methyl nucleotides.
DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2'-C-methylnucleotides and 10-23 DNAzymes containing (2'R) or (2'S)-...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia http://hdl.handle.net/20.500.12110/paper_17468272_v_n52_p521_Pontiggia |
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paper:paper_17468272_v_n52_p521_Pontiggia2023-06-08T16:28:26Z DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. 2' C methyluridine 2'-C-methyluridine deoxyribonucleotide deoxyribozyme drug derivative hammerhead ribozyme organophosphorus compound phosphoramidous acid ribonucleotide ribozyme uridine deoxyribonucleotide deoxyribozyme article chemistry synthesis Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2'-C-methylnucleotides and 10-23 DNAzymes containing (2'R) or (2'S)- 2'-C-methyl 2'-deoxynucleotides. In this work a new synthesis of 2'-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed.2'-C-methyl-2'-deoxynucleosides show differential preferred conformations depending on the configuration of the 2'-carbon. The influence of these modifications on the activity of 10-23 DNAzymes is also assessed. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia http://hdl.handle.net/20.500.12110/paper_17468272_v_n52_p521_Pontiggia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2' C methyluridine 2'-C-methyluridine deoxyribonucleotide deoxyribozyme drug derivative hammerhead ribozyme organophosphorus compound phosphoramidous acid ribonucleotide ribozyme uridine deoxyribonucleotide deoxyribozyme article chemistry synthesis Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine |
| spellingShingle |
2' C methyluridine 2'-C-methyluridine deoxyribonucleotide deoxyribozyme drug derivative hammerhead ribozyme organophosphorus compound phosphoramidous acid ribonucleotide ribozyme uridine deoxyribonucleotide deoxyribozyme article chemistry synthesis Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| topic_facet |
2' C methyluridine 2'-C-methyluridine deoxyribonucleotide deoxyribozyme drug derivative hammerhead ribozyme organophosphorus compound phosphoramidous acid ribonucleotide ribozyme uridine deoxyribonucleotide deoxyribozyme article chemistry synthesis Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine Deoxyribonucleotides DNA, Catalytic Organophosphorus Compounds Ribonucleotides RNA, Catalytic Uridine |
| description |
DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2'-C-methylnucleotides and 10-23 DNAzymes containing (2'R) or (2'S)- 2'-C-methyl 2'-deoxynucleotides. In this work a new synthesis of 2'-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed.2'-C-methyl-2'-deoxynucleosides show differential preferred conformations depending on the configuration of the 2'-carbon. The influence of these modifications on the activity of 10-23 DNAzymes is also assessed. |
| title |
DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| title_short |
DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| title_full |
DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| title_fullStr |
DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| title_full_unstemmed |
DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| title_sort |
dnazymes and ribozymes carrying 2'-c-methyl nucleotides. |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia http://hdl.handle.net/20.500.12110/paper_17468272_v_n52_p521_Pontiggia |
| _version_ |
1768542809164349440 |