GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
Introduction: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v17_n7_p511_Guerra http://hdl.handle.net/20.500.12110/paper_1699048X_v17_n7_p511_Guerra |
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paper:paper_1699048X_v17_n7_p511_Guerra2023-06-08T16:26:55Z GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes Adipocytes Breast mammary gland Cancer Cell lines Triglycerides enzyme galactose 3 O sulfotransferase 2 triacylglycerol unclassified drug CCAAT enhancer binding protein beta Fabp4 protein, mouse fatty acid binding protein GAL3ST2 protein, human Gal3St2 protein, mouse sulfotransferase sulfurtransferase triacylglycerol adipocyte animal cell Article breast epithelium cell differentiation column chromatography controlled study genetic transfection human human cell matrix assisted laser desorption ionization time of flight mass spectrometry mouse MTT assay nonhuman protein analysis protein expression two dimensional electrophoresis Western blotting 3T3 cell line adipocyte adipogenesis animal cell differentiation cytology female HEK293 cell line mass spectrometry MCF 7 cell line metabolism tumor cell line udder 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Cell Line, Tumor Fatty Acid-Binding Proteins Female HEK293 Cells Humans Mammary Glands, Animal MCF-7 Cells Mice NIH 3T3 Cells Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfotransferases Sulfurtransferases Triglycerides Introduction: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124–131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. Materials and methods: Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line. Results: We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells. Conclusions: Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity. © 2014, Federación de Sociedades Españolas de Oncología (FESEO). 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v17_n7_p511_Guerra http://hdl.handle.net/20.500.12110/paper_1699048X_v17_n7_p511_Guerra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adipocytes Breast mammary gland Cancer Cell lines Triglycerides enzyme galactose 3 O sulfotransferase 2 triacylglycerol unclassified drug CCAAT enhancer binding protein beta Fabp4 protein, mouse fatty acid binding protein GAL3ST2 protein, human Gal3St2 protein, mouse sulfotransferase sulfurtransferase triacylglycerol adipocyte animal cell Article breast epithelium cell differentiation column chromatography controlled study genetic transfection human human cell matrix assisted laser desorption ionization time of flight mass spectrometry mouse MTT assay nonhuman protein analysis protein expression two dimensional electrophoresis Western blotting 3T3 cell line adipocyte adipogenesis animal cell differentiation cytology female HEK293 cell line mass spectrometry MCF 7 cell line metabolism tumor cell line udder 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Cell Line, Tumor Fatty Acid-Binding Proteins Female HEK293 Cells Humans Mammary Glands, Animal MCF-7 Cells Mice NIH 3T3 Cells Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfotransferases Sulfurtransferases Triglycerides |
| spellingShingle |
Adipocytes Breast mammary gland Cancer Cell lines Triglycerides enzyme galactose 3 O sulfotransferase 2 triacylglycerol unclassified drug CCAAT enhancer binding protein beta Fabp4 protein, mouse fatty acid binding protein GAL3ST2 protein, human Gal3St2 protein, mouse sulfotransferase sulfurtransferase triacylglycerol adipocyte animal cell Article breast epithelium cell differentiation column chromatography controlled study genetic transfection human human cell matrix assisted laser desorption ionization time of flight mass spectrometry mouse MTT assay nonhuman protein analysis protein expression two dimensional electrophoresis Western blotting 3T3 cell line adipocyte adipogenesis animal cell differentiation cytology female HEK293 cell line mass spectrometry MCF 7 cell line metabolism tumor cell line udder 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Cell Line, Tumor Fatty Acid-Binding Proteins Female HEK293 Cells Humans Mammary Glands, Animal MCF-7 Cells Mice NIH 3T3 Cells Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfotransferases Sulfurtransferases Triglycerides GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes |
| topic_facet |
Adipocytes Breast mammary gland Cancer Cell lines Triglycerides enzyme galactose 3 O sulfotransferase 2 triacylglycerol unclassified drug CCAAT enhancer binding protein beta Fabp4 protein, mouse fatty acid binding protein GAL3ST2 protein, human Gal3St2 protein, mouse sulfotransferase sulfurtransferase triacylglycerol adipocyte animal cell Article breast epithelium cell differentiation column chromatography controlled study genetic transfection human human cell matrix assisted laser desorption ionization time of flight mass spectrometry mouse MTT assay nonhuman protein analysis protein expression two dimensional electrophoresis Western blotting 3T3 cell line adipocyte adipogenesis animal cell differentiation cytology female HEK293 cell line mass spectrometry MCF 7 cell line metabolism tumor cell line udder 3T3-L1 Cells Adipocytes Adipogenesis Animals CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Cell Line, Tumor Fatty Acid-Binding Proteins Female HEK293 Cells Humans Mammary Glands, Animal MCF-7 Cells Mice NIH 3T3 Cells Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfotransferases Sulfurtransferases Triglycerides |
| description |
Introduction: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124–131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. Materials and methods: Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line. Results: We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells. Conclusions: Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity. © 2014, Federación de Sociedades Españolas de Oncología (FESEO). |
| title |
GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes |
| title_short |
GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes |
| title_full |
GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes |
| title_fullStr |
GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes |
| title_full_unstemmed |
GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes |
| title_sort |
gal3st2 from mammary gland epithelial cells affects differentiation of 3t3-l1 preadipocytes |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v17_n7_p511_Guerra http://hdl.handle.net/20.500.12110/paper_1699048X_v17_n7_p511_Guerra |
| _version_ |
1768544523080695808 |