Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient

The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Wit...

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Autores principales:	Sigaut, Lorena, Colman Lerner, Alejandro Ariel, Ponce Dawson, Silvina
Publicado:	2014
Materias:	bicoid enhanced green fluorescent protein morphogen unclassified drug bicoid protein, Drosophila Drosophila protein homeodomain protein hybrid protein transactivator protein article binding site concentration (parameters) diffusion coefficient Drosophila embryo fluorescence correlation spectroscopy fluorescence recovery after photobleaching mathematical model molecular dynamics nonhuman validation process active transport animal biological model biology biophysics computer simulation diffusion Drosophila melanogaster embryology genetics metabolism morphogenesis spectrofluorometry transgenic animal Active Transport, Cell Nucleus Animals Animals, Genetically Modified Biophysical Phenomena Body Patterning Computational Biology Computer Simulation Diffusion Drosophila Proteins Fluorescence Recovery After Photobleaching Homeodomain Proteins Models, Biological Recombinant Fusion Proteins Spectrometry, Fluorescence Trans-Activators
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1553734X_v10_n6_p_Sigaut http://hdl.handle.net/20.500.12110/paper_1553734X_v10_n6_p_Sigaut
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1553734X_v10_n6_p_Sigaut
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spelling	paper:paper_1553734X_v10_n6_p_Sigaut2023-06-08T16:23:07Z Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient Sigaut, Lorena Colman Lerner, Alejandro Ariel Ponce Dawson, Silvina bicoid enhanced green fluorescent protein morphogen unclassified drug bicoid protein, Drosophila Drosophila protein homeodomain protein hybrid protein transactivator protein article binding site concentration (parameters) diffusion coefficient Drosophila embryo fluorescence correlation spectroscopy fluorescence recovery after photobleaching mathematical model molecular dynamics nonhuman validation process active transport animal biological model biology biophysics computer simulation diffusion Drosophila melanogaster embryology genetics metabolism morphogenesis spectrofluorometry transgenic animal Active Transport, Cell Nucleus Animals Animals, Genetically Modified Biophysical Phenomena Body Patterning Computational Biology Computer Simulation Diffusion Drosophila melanogaster Drosophila Proteins Fluorescence Recovery After Photobleaching Homeodomain Proteins Models, Biological Recombinant Fusion Proteins Spectrometry, Fluorescence Trans-Activators The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is "correct" what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times? In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times. © 2014 Sigaut et al. Fil:Sigaut, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ponce Dawson, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1553734X_v10_n6_p_Sigaut http://hdl.handle.net/20.500.12110/paper_1553734X_v10_n6_p_Sigaut
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bicoid enhanced green fluorescent protein morphogen unclassified drug bicoid protein, Drosophila Drosophila protein homeodomain protein hybrid protein transactivator protein article binding site concentration (parameters) diffusion coefficient Drosophila embryo fluorescence correlation spectroscopy fluorescence recovery after photobleaching mathematical model molecular dynamics nonhuman validation process active transport animal biological model biology biophysics computer simulation diffusion Drosophila melanogaster embryology genetics metabolism morphogenesis spectrofluorometry transgenic animal Active Transport, Cell Nucleus Animals Animals, Genetically Modified Biophysical Phenomena Body Patterning Computational Biology Computer Simulation Diffusion Drosophila melanogaster Drosophila Proteins Fluorescence Recovery After Photobleaching Homeodomain Proteins Models, Biological Recombinant Fusion Proteins Spectrometry, Fluorescence Trans-Activators
spellingShingle	bicoid enhanced green fluorescent protein morphogen unclassified drug bicoid protein, Drosophila Drosophila protein homeodomain protein hybrid protein transactivator protein article binding site concentration (parameters) diffusion coefficient Drosophila embryo fluorescence correlation spectroscopy fluorescence recovery after photobleaching mathematical model molecular dynamics nonhuman validation process active transport animal biological model biology biophysics computer simulation diffusion Drosophila melanogaster embryology genetics metabolism morphogenesis spectrofluorometry transgenic animal Active Transport, Cell Nucleus Animals Animals, Genetically Modified Biophysical Phenomena Body Patterning Computational Biology Computer Simulation Diffusion Drosophila melanogaster Drosophila Proteins Fluorescence Recovery After Photobleaching Homeodomain Proteins Models, Biological Recombinant Fusion Proteins Spectrometry, Fluorescence Trans-Activators Sigaut, Lorena Colman Lerner, Alejandro Ariel Ponce Dawson, Silvina Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
topic_facet	bicoid enhanced green fluorescent protein morphogen unclassified drug bicoid protein, Drosophila Drosophila protein homeodomain protein hybrid protein transactivator protein article binding site concentration (parameters) diffusion coefficient Drosophila embryo fluorescence correlation spectroscopy fluorescence recovery after photobleaching mathematical model molecular dynamics nonhuman validation process active transport animal biological model biology biophysics computer simulation diffusion Drosophila melanogaster embryology genetics metabolism morphogenesis spectrofluorometry transgenic animal Active Transport, Cell Nucleus Animals Animals, Genetically Modified Biophysical Phenomena Body Patterning Computational Biology Computer Simulation Diffusion Drosophila melanogaster Drosophila Proteins Fluorescence Recovery After Photobleaching Homeodomain Proteins Models, Biological Recombinant Fusion Proteins Spectrometry, Fluorescence Trans-Activators
description	The gradient of Bicoid (Bcd) is key for the establishment of the anterior-posterior axis in Drosophila embryos. The gradient properties are compatible with the SDD model in which Bcd is synthesized at the anterior pole and then diffuses into the embryo and is degraded with a characteristic time. Within this model, the Bcd diffusion coefficient is critical to set the timescale of gradient formation. This coefficient has been measured using two optical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS), obtaining estimates in which the FCS value is an order of magnitude larger than the FRAP one. This discrepancy raises the following questions: which estimate is "correct" what is the reason for the disparity; and can the SDD model explain Bcd gradient formation within the experimentally observed times? In this paper, we use a simple biophysical model in which Bcd diffuses and interacts with binding sites to show that both the FRAP and the FCS estimates may be correct and compatible with the observed timescale of gradient formation. The discrepancy arises from the fact that FCS and FRAP report on different effective (concentration dependent) diffusion coefficients, one of which describes the spreading rate of the individual Bcd molecules (the messengers) and the other one that of their concentration (the message). The latter is the one that is more relevant for the gradient establishment and is compatible with its formation within the experimentally observed times. © 2014 Sigaut et al.
author	Sigaut, Lorena Colman Lerner, Alejandro Ariel Ponce Dawson, Silvina
author_facet	Sigaut, Lorena Colman Lerner, Alejandro Ariel Ponce Dawson, Silvina
author_sort	Sigaut, Lorena
title	Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
title_short	Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
title_full	Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
title_fullStr	Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
title_full_unstemmed	Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient
title_sort	messages do diffuse faster than messengers: reconciling disparate estimates of the morphogen bicoid diffusion coefficient
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1553734X_v10_n6_p_Sigaut http://hdl.handle.net/20.500.12110/paper_1553734X_v10_n6_p_Sigaut
work_keys_str_mv	AT sigautlorena messagesdodiffusefasterthanmessengersreconcilingdisparateestimatesofthemorphogenbicoiddiffusioncoefficient AT colmanlerneralejandroariel messagesdodiffusefasterthanmessengersreconcilingdisparateestimatesofthemorphogenbicoiddiffusioncoefficient AT poncedawsonsilvina messagesdodiffusefasterthanmessengersreconcilingdisparateestimatesofthemorphogenbicoiddiffusioncoefficient
_version_	1768544011112415232

Messages Do Diffuse Faster than Messengers: Reconciling Disparate Estimates of the Morphogen Bicoid Diffusion Coefficient

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