Reduced nitric oxide synthase and cyclo-oxygenase activity in the uterus of non-obese diabetic mice

A functional interaction between progesterone, Th2 cytokines and a suitable balance between nitric oxide and prostaglandins in the uterus is considered to have a major role in the success of embryo implantation and pregnancy. Non-obese diabetic (NOD) mice offer a suitable model to study the modulato...

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Detalles Bibliográficos
Autores principales: Roca, Valeria Inés, Larocca, Luciana, Calafat, Mario José, Aisemberg, Julieta, Franchi, Ana María, Pérez Leirós, Claudia
Publicado: 2006
Materias:
cytokine
gamma interferon
interleukin 10
interleukin 12
neuropeptide
nitric oxide
nitric oxide synthase
progesterone
prostaglandin
prostaglandin synthase
tumor necrosis factor alpha
vasoactive intestinal polypeptide
animal experiment
animal model
animal tissue
article
autoimmunity
controlled study
diabetes mellitus
enzyme activity
exocrine gland
female
impaired glucose tolerance
mouse
nidation
nonhuman
pregnancy
priority journal
proestrus
protein interaction
signal transduction
Sjoegren syndrome
smooth muscle relaxation
Th1 cell
Th2 cell
uterus
vasodilatation
Animals
Cyclooxygenase Inhibitors
Diabetes Mellitus, Type 1
Dinoprostone
Enzyme Activation
Female
Immunohistochemistry
Indomethacin
Interferon Type II
Interleukin-10
Interleukin-12
Mice
Mice, Inbred BALB C
Mice, Inbred NOD
Models, Animal
Nitric Oxide Synthase
omega-N-Methylarginine
Pregnancy
Prostaglandin-Endoperoxide Synthases
Sjogren's Syndrome
Th1 Cells
Tumor Necrosis Factor-alpha
Uterus
Vasoactive Intestinal Peptide
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14701626_v132_n6_p931_Roca
http://hdl.handle.net/20.500.12110/paper_14701626_v132_n6_p931_Roca
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:A functional interaction between progesterone, Th2 cytokines and a suitable balance between nitric oxide and prostaglandins in the uterus is considered to have a major role in the success of embryo implantation and pregnancy. Non-obese diabetic (NOD) mice offer a suitable model to study the modulatory role of Th1 cytokines on uterus signalling and function, since at the prediabetic stage they develop a spontaneous Th1 autoimmune response against exocrine glands similar to Sjögren's syndrome. Vasoactive intestinal peptide (VIP) is a vasoactive neuro- and immunopeptide that promotes Th2 profiles and contributes to the smooth muscle relaxation and vasodilation. The aim of the present study was to investigate the activities of nitric oxide synthase and cyclo-oxygenase and the effect of VIP in the uterus of NOD mice with an emerging Th1 cytokine response. We present evidence of a reduced basal and VIP-stimulated activity of both enzymes in the uterus of NOD mice compared with normal BALB/c mice in proestrus. An altered functional interaction between both enzymes is also present in NOD mice at the time when increased levels of serum interleukin (IL)-12 and tumour necrosis factor-α but not interferon (IFN)-γ or IL-10 were detected. We conclude that signalling alterations in uteri of NOD mice are simultaneous to the onset of a systemic Th1 cytokine response. © 2006 Society for Reproduction and Fertility.

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