Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System

For brown howler monkeys (Alouatta guariba clamitans), diploid chromosome numbers varying from 2n = 45 to 2n = 52, with XX/XY, X1X1X2X2/X1X2Y, and X1X1X2X2X3X3/X1X2X3Y1Y2 sex chromosome systems have been described by mitotic studies but still await confirmation by meiotic analyses. We analyzed 3 mal...

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Autores principales:	Steinberg, Eliana Ruth, Rossi, Luis Francisco, Mudry, Marta Dolores
Publicado:	2017
Materias:	Alouatta guariba clamitans Meiosis Multiple sex chromosome systems Sex chromosome pentavalent adult Alouatta animal experiment animal tissue Argentina Article C banding centromere chromosome deletion chromosome G band chromosome number chromosome pairing cytogenetics diploidy female geographic distribution immunodetection karyotype male metaphase nonhuman pachytene priority journal prophase somatic cell spermatocyte synaptonemal complex testis biopsy X chromosome Y chromosome animal chromosome analysis cytology genetics germ cell meiosis physiology sex chromosome sex determination process veterinary Animals Cytogenetic Analysis Female Germ Cells Male Sex Chromosomes Sex Determination Processes Spermatocytes
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v151_n3_p131_Steinberg http://hdl.handle.net/20.500.12110/paper_14248581_v151_n3_p131_Steinberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14248581_v151_n3_p131_Steinberg
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spelling	paper:paper_14248581_v151_n3_p131_Steinberg2023-06-08T16:14:03Z Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System Steinberg, Eliana Ruth Rossi, Luis Francisco Mudry, Marta Dolores Alouatta guariba clamitans Meiosis Multiple sex chromosome systems Sex chromosome pentavalent adult Alouatta Alouatta guariba clamitans animal experiment animal tissue Argentina Article C banding centromere chromosome deletion chromosome G band chromosome number chromosome pairing cytogenetics diploidy female geographic distribution immunodetection karyotype male metaphase nonhuman pachytene priority journal prophase somatic cell spermatocyte synaptonemal complex testis biopsy X chromosome Y chromosome Alouatta animal chromosome analysis cytology genetics germ cell meiosis physiology sex chromosome sex determination process veterinary Alouatta Animals Cytogenetic Analysis Female Germ Cells Male Meiosis Sex Chromosomes Sex Determination Processes Spermatocytes For brown howler monkeys (Alouatta guariba clamitans), diploid chromosome numbers varying from 2n = 45 to 2n = 52, with XX/XY, X1X1X2X2/X1X2Y, and X1X1X2X2X3X3/X1X2X3Y1Y2 sex chromosome systems have been described by mitotic studies but still await confirmation by meiotic analyses. We analyzed 3 male individuals sampled in the wild (in the municipality of Santa Maria, RS, Brazil) as well as 1 male and 1 female individual in captivity at the Saõ Braz breeding center. Peripheral blood samples and testicular biopsies were taken. We found different diploid numbers for both sexes in somatic cells, 2n = 45,X1X2X3Y1Y2 in males and 2n = 46,X1X1X2X2X3X3 in females, with 4 metacentric (9-12), 7 submetacentric (1-6, 8), and 9 acrocentric autosomal chromosome pairs (13-20, 22). X1 and X2 were submetacentric chromosomes, while X3, Y1, and Y2 were acrocentric ones. Spermatocyte microspreads were examined for synaptonemal complexes. Pachytene spermatocyte analysis was done to verify the chromosome number and morphologies observed in mitotic karyotypes. Immunodetection was performed using anti-SMC3 and anti-CREST antibodies. The presence of a sex chromosome pentavalent X1X2X3Y1Y2 in the males was confirmed by C-banding in metaphase I and by immunodetection in prophase I by the clear identification of 5 centromeres. The G-banded karyotype corresponded to that previously described for A. g. clamitans in the south of Brazil (Curitiba, Parana State, and Blumenau, Santa Catarina State) and for the Misiones Province, Argentina. © 2017 S. Karger AG, Basel. Fil:Steinberg, E.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, L.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v151_n3_p131_Steinberg http://hdl.handle.net/20.500.12110/paper_14248581_v151_n3_p131_Steinberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alouatta guariba clamitans Meiosis Multiple sex chromosome systems Sex chromosome pentavalent adult Alouatta Alouatta guariba clamitans animal experiment animal tissue Argentina Article C banding centromere chromosome deletion chromosome G band chromosome number chromosome pairing cytogenetics diploidy female geographic distribution immunodetection karyotype male metaphase nonhuman pachytene priority journal prophase somatic cell spermatocyte synaptonemal complex testis biopsy X chromosome Y chromosome Alouatta animal chromosome analysis cytology genetics germ cell meiosis physiology sex chromosome sex determination process veterinary Alouatta Animals Cytogenetic Analysis Female Germ Cells Male Meiosis Sex Chromosomes Sex Determination Processes Spermatocytes
spellingShingle	Alouatta guariba clamitans Meiosis Multiple sex chromosome systems Sex chromosome pentavalent adult Alouatta Alouatta guariba clamitans animal experiment animal tissue Argentina Article C banding centromere chromosome deletion chromosome G band chromosome number chromosome pairing cytogenetics diploidy female geographic distribution immunodetection karyotype male metaphase nonhuman pachytene priority journal prophase somatic cell spermatocyte synaptonemal complex testis biopsy X chromosome Y chromosome Alouatta animal chromosome analysis cytology genetics germ cell meiosis physiology sex chromosome sex determination process veterinary Alouatta Animals Cytogenetic Analysis Female Germ Cells Male Meiosis Sex Chromosomes Sex Determination Processes Spermatocytes Steinberg, Eliana Ruth Rossi, Luis Francisco Mudry, Marta Dolores Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
topic_facet	Alouatta guariba clamitans Meiosis Multiple sex chromosome systems Sex chromosome pentavalent adult Alouatta Alouatta guariba clamitans animal experiment animal tissue Argentina Article C banding centromere chromosome deletion chromosome G band chromosome number chromosome pairing cytogenetics diploidy female geographic distribution immunodetection karyotype male metaphase nonhuman pachytene priority journal prophase somatic cell spermatocyte synaptonemal complex testis biopsy X chromosome Y chromosome Alouatta animal chromosome analysis cytology genetics germ cell meiosis physiology sex chromosome sex determination process veterinary Alouatta Animals Cytogenetic Analysis Female Germ Cells Male Meiosis Sex Chromosomes Sex Determination Processes Spermatocytes
description	For brown howler monkeys (Alouatta guariba clamitans), diploid chromosome numbers varying from 2n = 45 to 2n = 52, with XX/XY, X1X1X2X2/X1X2Y, and X1X1X2X2X3X3/X1X2X3Y1Y2 sex chromosome systems have been described by mitotic studies but still await confirmation by meiotic analyses. We analyzed 3 male individuals sampled in the wild (in the municipality of Santa Maria, RS, Brazil) as well as 1 male and 1 female individual in captivity at the Saõ Braz breeding center. Peripheral blood samples and testicular biopsies were taken. We found different diploid numbers for both sexes in somatic cells, 2n = 45,X1X2X3Y1Y2 in males and 2n = 46,X1X1X2X2X3X3 in females, with 4 metacentric (9-12), 7 submetacentric (1-6, 8), and 9 acrocentric autosomal chromosome pairs (13-20, 22). X1 and X2 were submetacentric chromosomes, while X3, Y1, and Y2 were acrocentric ones. Spermatocyte microspreads were examined for synaptonemal complexes. Pachytene spermatocyte analysis was done to verify the chromosome number and morphologies observed in mitotic karyotypes. Immunodetection was performed using anti-SMC3 and anti-CREST antibodies. The presence of a sex chromosome pentavalent X1X2X3Y1Y2 in the males was confirmed by C-banding in metaphase I and by immunodetection in prophase I by the clear identification of 5 centromeres. The G-banded karyotype corresponded to that previously described for A. g. clamitans in the south of Brazil (Curitiba, Parana State, and Blumenau, Santa Catarina State) and for the Misiones Province, Argentina. © 2017 S. Karger AG, Basel.
author	Steinberg, Eliana Ruth Rossi, Luis Francisco Mudry, Marta Dolores
author_facet	Steinberg, Eliana Ruth Rossi, Luis Francisco Mudry, Marta Dolores
author_sort	Steinberg, Eliana Ruth
title	Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
title_short	Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
title_full	Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
title_fullStr	Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
title_full_unstemmed	Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System
title_sort	cytogenetic characterization of brown howler monkeys, alouatta guariba clamitans (atelidae, platyrrhini): meiotic confirmation of an x1x1x2x2x3x3/x1x2x3y1y2 sex chromosome system
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14248581_v151_n3_p131_Steinberg http://hdl.handle.net/20.500.12110/paper_14248581_v151_n3_p131_Steinberg
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Cytogenetic Characterization of Brown Howler Monkeys, Alouatta guariba clamitans (Atelidae, Platyrrhini): Meiotic Confirmation of an X1X1X2X2X3X3/X1X2X3Y1Y2 Sex Chromosome System

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