N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes

Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our...

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Autores principales:	Calzadilla, Pablo Ignacio, Sapochnik, Daiana Andrea, Cosentino, María Soledad, Diz, Virginia Emilse, Dicelio, Lelia Elina, Calvo, Juan Carlos, Guerra, Liliana Noemí
Publicado:	2011
Materias:	Adipocyte differentiation NAC Triglyceride acetylcysteine beta actin CCAAT enhancer binding protein copper zinc superoxide dismutase glutathione peroxidase glutathione peroxidase 1 insulin receptor manganese superoxide dismutase metallothionein peroxisome proliferator activated receptor gamma protein kinase B reactive oxygen metabolite somatomedin C superoxide dismutase triacylglycerol biological marker CCAAT enhancer binding protein beta glutathione adipocyte aerobic metabolism article cell culture cell differentiation concentration response controlled study culture medium DNA content down regulation enzyme activity enzyme assay human human cell lipid peroxidation lipid storage low drug dose obesity oxidative stress protein content protein expression protein phosphorylation Western blotting 3T3 cell line animal cytology metabolism mouse Mus 3T3-L1 Cells Acetylcysteine Adipocytes Animals Biological Markers CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Glutathione Glutathione Peroxidase Mice PPAR gamma Reactive Oxygen Species Superoxide Dismutase Triglycerides
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14220067_v12_n10_p6936_Calzadilla http://hdl.handle.net/20.500.12110/paper_14220067_v12_n10_p6936_Calzadilla
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_14220067_v12_n10_p6936_Calzadilla
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spelling	paper:paper_14220067_v12_n10_p6936_Calzadilla2023-06-08T16:13:48Z N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes Calzadilla, Pablo Ignacio Sapochnik, Daiana Andrea Cosentino, María Soledad Diz, Virginia Emilse Dicelio, Lelia Elina Calvo, Juan Carlos Guerra, Liliana Noemí Adipocyte differentiation NAC Triglyceride acetylcysteine beta actin CCAAT enhancer binding protein copper zinc superoxide dismutase glutathione peroxidase glutathione peroxidase 1 insulin receptor manganese superoxide dismutase metallothionein peroxisome proliferator activated receptor gamma protein kinase B reactive oxygen metabolite somatomedin C superoxide dismutase triacylglycerol acetylcysteine biological marker CCAAT enhancer binding protein beta glutathione glutathione peroxidase peroxisome proliferator activated receptor gamma reactive oxygen metabolite superoxide dismutase triacylglycerol adipocyte aerobic metabolism article cell culture cell differentiation concentration response controlled study culture medium DNA content down regulation enzyme activity enzyme assay human human cell lipid peroxidation lipid storage low drug dose obesity oxidative stress protein content protein expression protein phosphorylation Western blotting 3T3 cell line adipocyte animal cell differentiation cytology metabolism mouse Mus 3T3-L1 Cells Acetylcysteine Adipocytes Animals Biological Markers CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Glutathione Glutathione Peroxidase Mice PPAR gamma Reactive Oxygen Species Superoxide Dismutase Triglycerides Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects. © 2011 by the authors; licensee MDPI, Basel, Switzerland. Fil:Calzadilla, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sapochnik, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cosentino, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Diz, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dicelio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14220067_v12_n10_p6936_Calzadilla http://hdl.handle.net/20.500.12110/paper_14220067_v12_n10_p6936_Calzadilla
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adipocyte differentiation NAC Triglyceride acetylcysteine beta actin CCAAT enhancer binding protein copper zinc superoxide dismutase glutathione peroxidase glutathione peroxidase 1 insulin receptor manganese superoxide dismutase metallothionein peroxisome proliferator activated receptor gamma protein kinase B reactive oxygen metabolite somatomedin C superoxide dismutase triacylglycerol acetylcysteine biological marker CCAAT enhancer binding protein beta glutathione glutathione peroxidase peroxisome proliferator activated receptor gamma reactive oxygen metabolite superoxide dismutase triacylglycerol adipocyte aerobic metabolism article cell culture cell differentiation concentration response controlled study culture medium DNA content down regulation enzyme activity enzyme assay human human cell lipid peroxidation lipid storage low drug dose obesity oxidative stress protein content protein expression protein phosphorylation Western blotting 3T3 cell line adipocyte animal cell differentiation cytology metabolism mouse Mus 3T3-L1 Cells Acetylcysteine Adipocytes Animals Biological Markers CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Glutathione Glutathione Peroxidase Mice PPAR gamma Reactive Oxygen Species Superoxide Dismutase Triglycerides
spellingShingle	Adipocyte differentiation NAC Triglyceride acetylcysteine beta actin CCAAT enhancer binding protein copper zinc superoxide dismutase glutathione peroxidase glutathione peroxidase 1 insulin receptor manganese superoxide dismutase metallothionein peroxisome proliferator activated receptor gamma protein kinase B reactive oxygen metabolite somatomedin C superoxide dismutase triacylglycerol acetylcysteine biological marker CCAAT enhancer binding protein beta glutathione glutathione peroxidase peroxisome proliferator activated receptor gamma reactive oxygen metabolite superoxide dismutase triacylglycerol adipocyte aerobic metabolism article cell culture cell differentiation concentration response controlled study culture medium DNA content down regulation enzyme activity enzyme assay human human cell lipid peroxidation lipid storage low drug dose obesity oxidative stress protein content protein expression protein phosphorylation Western blotting 3T3 cell line adipocyte animal cell differentiation cytology metabolism mouse Mus 3T3-L1 Cells Acetylcysteine Adipocytes Animals Biological Markers CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Glutathione Glutathione Peroxidase Mice PPAR gamma Reactive Oxygen Species Superoxide Dismutase Triglycerides Calzadilla, Pablo Ignacio Sapochnik, Daiana Andrea Cosentino, María Soledad Diz, Virginia Emilse Dicelio, Lelia Elina Calvo, Juan Carlos Guerra, Liliana Noemí N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
topic_facet	Adipocyte differentiation NAC Triglyceride acetylcysteine beta actin CCAAT enhancer binding protein copper zinc superoxide dismutase glutathione peroxidase glutathione peroxidase 1 insulin receptor manganese superoxide dismutase metallothionein peroxisome proliferator activated receptor gamma protein kinase B reactive oxygen metabolite somatomedin C superoxide dismutase triacylglycerol acetylcysteine biological marker CCAAT enhancer binding protein beta glutathione glutathione peroxidase peroxisome proliferator activated receptor gamma reactive oxygen metabolite superoxide dismutase triacylglycerol adipocyte aerobic metabolism article cell culture cell differentiation concentration response controlled study culture medium DNA content down regulation enzyme activity enzyme assay human human cell lipid peroxidation lipid storage low drug dose obesity oxidative stress protein content protein expression protein phosphorylation Western blotting 3T3 cell line adipocyte animal cell differentiation cytology metabolism mouse Mus 3T3-L1 Cells Acetylcysteine Adipocytes Animals Biological Markers CCAAT-Enhancer-Binding Protein-beta Cell Differentiation Glutathione Glutathione Peroxidase Mice PPAR gamma Reactive Oxygen Species Superoxide Dismutase Triglycerides
description	Oxidative stress plays a critical role in the pathogenesis of diabetes, hypertension and atherosclerosis. Some authors reported that fat accumulation correlates to systemic oxidative stress in humans and mice, but the relationship of lipid production and oxidative metabolism is still unclear. In our laboratory we used 3T3-L1 preadipocytes, which are able to differentiate into mature adipocytes and accumulate lipids, as obesity model. We showed that intracellular reactive oxygen species (ROS) and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in parallel with fat accumulation. Meanwhile N-acetylcysteine (NAC), a well known antioxidant and Glutathione (GSH) precursor, inhibited ROS levels as well as fat accumulation in a concentration-dependent manner. NAC also inhibited both adipogenic transcription factors CCAAT/enhancer binding protein beta (C/EBP) and peroxisomal proliferator activated receptor gamma (PPAR β) expression; we suggested that intracellular GSH content could be responsible for these effects. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
author	Calzadilla, Pablo Ignacio Sapochnik, Daiana Andrea Cosentino, María Soledad Diz, Virginia Emilse Dicelio, Lelia Elina Calvo, Juan Carlos Guerra, Liliana Noemí
author_facet	Calzadilla, Pablo Ignacio Sapochnik, Daiana Andrea Cosentino, María Soledad Diz, Virginia Emilse Dicelio, Lelia Elina Calvo, Juan Carlos Guerra, Liliana Noemí
author_sort	Calzadilla, Pablo Ignacio
title	N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_short	N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_full	N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_fullStr	N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_full_unstemmed	N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes
title_sort	n-acetylcysteine reduces markers of differentiation in 3t3-l1 adipocytes
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14220067_v12_n10_p6936_Calzadilla http://hdl.handle.net/20.500.12110/paper_14220067_v12_n10_p6936_Calzadilla
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N-acetylcysteine reduces markers of differentiation in 3T3-L1 adipocytes

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