Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour
Fibroblast proliferation is a key process in tissue remodeling and mast cells (MCs) are thought to play a crucial role. Having established that the three major MC products, tryptase, histamine and TNF-alpha (TNF) are normally present in human skin MCs, which are in close proximity to dermal fibrobla...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1420682X_v62_n23_p2867_Albrecht http://hdl.handle.net/20.500.12110/paper_1420682X_v62_n23_p2867_Albrecht |
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paper:paper_1420682X_v62_n23_p2867_Albrecht2023-06-08T16:13:46Z Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour Calcium Histamine Mast cell Proliferation Tissue remodeling TNF-alpha Tryptase calcium ion histamine proteinase activated receptor 2 tryptase tumor necrosis factor alpha tumor necrosis factor receptor 1 tumor necrosis factor receptor 2 article calcium cell level cell cycle cell proliferation cell stimulation controlled study fetus fibroblast gene expression human human cell immunohistochemistry mast cell polymerase chain reaction protein expression skin fibroblast Cell Cycle Cell Proliferation Cells, Cultured Fibroblasts Gene Expression Histamine Humans Mast Cells Peptides Receptor, PAR-2 Reference Values Serine Endopeptidases Skin Time Factors Tryptases Tumor Necrosis Factor-alpha Fibroblast proliferation is a key process in tissue remodeling and mast cells (MCs) are thought to play a crucial role. Having established that the three major MC products, tryptase, histamine and TNF-alpha (TNF) are normally present in human skin MCs, which are in close proximity to dermal fibroblasts, we studied their individual effects on cell cycle-controlled human dermal fibroblasts (HFFF2). These cells express receptors (H1, PAR2, TNFR1/2) for the major MC mediators, but only tryptase or a PAR2 agonist peptide stimulated proliferation and gene expression. TNF was antimitotic, and histamine, while elevating intracellular Ca2+ levels at high concentrations, did not affect proliferation. We conclude that MC products but also composition and numbers of respective receptors on fibroblasts are crucially responsible for fibroproliferative events. © Birkhäuser Verlag, 2005. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1420682X_v62_n23_p2867_Albrecht http://hdl.handle.net/20.500.12110/paper_1420682X_v62_n23_p2867_Albrecht |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium Histamine Mast cell Proliferation Tissue remodeling TNF-alpha Tryptase calcium ion histamine proteinase activated receptor 2 tryptase tumor necrosis factor alpha tumor necrosis factor receptor 1 tumor necrosis factor receptor 2 article calcium cell level cell cycle cell proliferation cell stimulation controlled study fetus fibroblast gene expression human human cell immunohistochemistry mast cell polymerase chain reaction protein expression skin fibroblast Cell Cycle Cell Proliferation Cells, Cultured Fibroblasts Gene Expression Histamine Humans Mast Cells Peptides Receptor, PAR-2 Reference Values Serine Endopeptidases Skin Time Factors Tryptases Tumor Necrosis Factor-alpha |
| spellingShingle |
Calcium Histamine Mast cell Proliferation Tissue remodeling TNF-alpha Tryptase calcium ion histamine proteinase activated receptor 2 tryptase tumor necrosis factor alpha tumor necrosis factor receptor 1 tumor necrosis factor receptor 2 article calcium cell level cell cycle cell proliferation cell stimulation controlled study fetus fibroblast gene expression human human cell immunohistochemistry mast cell polymerase chain reaction protein expression skin fibroblast Cell Cycle Cell Proliferation Cells, Cultured Fibroblasts Gene Expression Histamine Humans Mast Cells Peptides Receptor, PAR-2 Reference Values Serine Endopeptidases Skin Time Factors Tryptases Tumor Necrosis Factor-alpha Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour |
| topic_facet |
Calcium Histamine Mast cell Proliferation Tissue remodeling TNF-alpha Tryptase calcium ion histamine proteinase activated receptor 2 tryptase tumor necrosis factor alpha tumor necrosis factor receptor 1 tumor necrosis factor receptor 2 article calcium cell level cell cycle cell proliferation cell stimulation controlled study fetus fibroblast gene expression human human cell immunohistochemistry mast cell polymerase chain reaction protein expression skin fibroblast Cell Cycle Cell Proliferation Cells, Cultured Fibroblasts Gene Expression Histamine Humans Mast Cells Peptides Receptor, PAR-2 Reference Values Serine Endopeptidases Skin Time Factors Tryptases Tumor Necrosis Factor-alpha |
| description |
Fibroblast proliferation is a key process in tissue remodeling and mast cells (MCs) are thought to play a crucial role. Having established that the three major MC products, tryptase, histamine and TNF-alpha (TNF) are normally present in human skin MCs, which are in close proximity to dermal fibroblasts, we studied their individual effects on cell cycle-controlled human dermal fibroblasts (HFFF2). These cells express receptors (H1, PAR2, TNFR1/2) for the major MC mediators, but only tryptase or a PAR2 agonist peptide stimulated proliferation and gene expression. TNF was antimitotic, and histamine, while elevating intracellular Ca2+ levels at high concentrations, did not affect proliferation. We conclude that MC products but also composition and numbers of respective receptors on fibroblasts are crucially responsible for fibroproliferative events. © Birkhäuser Verlag, 2005. |
| title |
Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour |
| title_short |
Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour |
| title_full |
Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour |
| title_fullStr |
Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour |
| title_full_unstemmed |
Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour |
| title_sort |
divergent effects of the major mast cell products histamine, tryptase and tnf-alpha on human fibroblast behaviour |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1420682X_v62_n23_p2867_Albrecht http://hdl.handle.net/20.500.12110/paper_1420682X_v62_n23_p2867_Albrecht |
| _version_ |
1768542753526906880 |