Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.
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2000
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14203049_v5_n3_p535_Rogert http://hdl.handle.net/20.500.12110/paper_14203049_v5_n3_p535_Rogert |
Aporte de: |
Sumario: | E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine. |
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