Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be...
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paper:paper_13693786_v53_n7_p699_Pena2023-06-08T16:12:13Z Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers animal feeds feed-borne A. fumigatus genetic variability estimation molecular typing molecular marker Argentina Article Aspergillus fumigatus Brazil fungal biomass fungal strain genetic similarity genetic variability geographic origin nonhuman nucleotide sequence polymerase chain reaction random amplified polymorphic DNA restriction fragment length polymorphism animal aspergillosis Aspergillus fumigatus classification cluster analysis DNA fingerprinting DNA sequence food control genetic variation genotype human isolation and purification microbiological examination microbiology molecular epidemiology molecular genetics molecular typing phylogeography veterinary Animals Argentina Aspergillosis Aspergillus fumigatus Brazil Cluster Analysis DNA Fingerprinting Food Microbiology Genetic Variation Genotype Humans Molecular Epidemiology Molecular Sequence Data Molecular Typing Mycological Typing Techniques Phylogeography Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Sequence Analysis, DNA Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed. © 2015 The Author. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13693786_v53_n7_p699_Pena http://hdl.handle.net/20.500.12110/paper_13693786_v53_n7_p699_Pena |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
animal feeds feed-borne A. fumigatus genetic variability estimation molecular typing molecular marker Argentina Article Aspergillus fumigatus Brazil fungal biomass fungal strain genetic similarity genetic variability geographic origin nonhuman nucleotide sequence polymerase chain reaction random amplified polymorphic DNA restriction fragment length polymorphism animal aspergillosis Aspergillus fumigatus classification cluster analysis DNA fingerprinting DNA sequence food control genetic variation genotype human isolation and purification microbiological examination microbiology molecular epidemiology molecular genetics molecular typing phylogeography veterinary Animals Argentina Aspergillosis Aspergillus fumigatus Brazil Cluster Analysis DNA Fingerprinting Food Microbiology Genetic Variation Genotype Humans Molecular Epidemiology Molecular Sequence Data Molecular Typing Mycological Typing Techniques Phylogeography Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Sequence Analysis, DNA |
spellingShingle |
animal feeds feed-borne A. fumigatus genetic variability estimation molecular typing molecular marker Argentina Article Aspergillus fumigatus Brazil fungal biomass fungal strain genetic similarity genetic variability geographic origin nonhuman nucleotide sequence polymerase chain reaction random amplified polymorphic DNA restriction fragment length polymorphism animal aspergillosis Aspergillus fumigatus classification cluster analysis DNA fingerprinting DNA sequence food control genetic variation genotype human isolation and purification microbiological examination microbiology molecular epidemiology molecular genetics molecular typing phylogeography veterinary Animals Argentina Aspergillosis Aspergillus fumigatus Brazil Cluster Analysis DNA Fingerprinting Food Microbiology Genetic Variation Genotype Humans Molecular Epidemiology Molecular Sequence Data Molecular Typing Mycological Typing Techniques Phylogeography Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Sequence Analysis, DNA Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers |
topic_facet |
animal feeds feed-borne A. fumigatus genetic variability estimation molecular typing molecular marker Argentina Article Aspergillus fumigatus Brazil fungal biomass fungal strain genetic similarity genetic variability geographic origin nonhuman nucleotide sequence polymerase chain reaction random amplified polymorphic DNA restriction fragment length polymorphism animal aspergillosis Aspergillus fumigatus classification cluster analysis DNA fingerprinting DNA sequence food control genetic variation genotype human isolation and purification microbiological examination microbiology molecular epidemiology molecular genetics molecular typing phylogeography veterinary Animals Argentina Aspergillosis Aspergillus fumigatus Brazil Cluster Analysis DNA Fingerprinting Food Microbiology Genetic Variation Genotype Humans Molecular Epidemiology Molecular Sequence Data Molecular Typing Mycological Typing Techniques Phylogeography Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Sequence Analysis, DNA |
description |
Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed. © 2015 The Author. |
title |
Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers |
title_short |
Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers |
title_full |
Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers |
title_fullStr |
Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers |
title_full_unstemmed |
Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers |
title_sort |
characterization and genetic variability of feed-borne and clinical animal/human aspergillus fumigatus strains using molecular markers |
publishDate |
2015 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13693786_v53_n7_p699_Pena http://hdl.handle.net/20.500.12110/paper_13693786_v53_n7_p699_Pena |
_version_ |
1768541766260097024 |