Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers

Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be...

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Publicado: 2015
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13693786_v53_n7_p699_Pena
http://hdl.handle.net/20.500.12110/paper_13693786_v53_n7_p699_Pena
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spelling paper:paper_13693786_v53_n7_p699_Pena2023-06-08T16:12:13Z Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers animal feeds feed-borne A. fumigatus genetic variability estimation molecular typing molecular marker Argentina Article Aspergillus fumigatus Brazil fungal biomass fungal strain genetic similarity genetic variability geographic origin nonhuman nucleotide sequence polymerase chain reaction random amplified polymorphic DNA restriction fragment length polymorphism animal aspergillosis Aspergillus fumigatus classification cluster analysis DNA fingerprinting DNA sequence food control genetic variation genotype human isolation and purification microbiological examination microbiology molecular epidemiology molecular genetics molecular typing phylogeography veterinary Animals Argentina Aspergillosis Aspergillus fumigatus Brazil Cluster Analysis DNA Fingerprinting Food Microbiology Genetic Variation Genotype Humans Molecular Epidemiology Molecular Sequence Data Molecular Typing Mycological Typing Techniques Phylogeography Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Sequence Analysis, DNA Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed. © 2015 The Author. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13693786_v53_n7_p699_Pena http://hdl.handle.net/20.500.12110/paper_13693786_v53_n7_p699_Pena
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic animal feeds
feed-borne A. fumigatus
genetic variability estimation
molecular typing
molecular marker
Argentina
Article
Aspergillus fumigatus
Brazil
fungal biomass
fungal strain
genetic similarity
genetic variability
geographic origin
nonhuman
nucleotide sequence
polymerase chain reaction
random amplified polymorphic DNA
restriction fragment length polymorphism
animal
aspergillosis
Aspergillus fumigatus
classification
cluster analysis
DNA fingerprinting
DNA sequence
food control
genetic variation
genotype
human
isolation and purification
microbiological examination
microbiology
molecular epidemiology
molecular genetics
molecular typing
phylogeography
veterinary
Animals
Argentina
Aspergillosis
Aspergillus fumigatus
Brazil
Cluster Analysis
DNA Fingerprinting
Food Microbiology
Genetic Variation
Genotype
Humans
Molecular Epidemiology
Molecular Sequence Data
Molecular Typing
Mycological Typing Techniques
Phylogeography
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
spellingShingle animal feeds
feed-borne A. fumigatus
genetic variability estimation
molecular typing
molecular marker
Argentina
Article
Aspergillus fumigatus
Brazil
fungal biomass
fungal strain
genetic similarity
genetic variability
geographic origin
nonhuman
nucleotide sequence
polymerase chain reaction
random amplified polymorphic DNA
restriction fragment length polymorphism
animal
aspergillosis
Aspergillus fumigatus
classification
cluster analysis
DNA fingerprinting
DNA sequence
food control
genetic variation
genotype
human
isolation and purification
microbiological examination
microbiology
molecular epidemiology
molecular genetics
molecular typing
phylogeography
veterinary
Animals
Argentina
Aspergillosis
Aspergillus fumigatus
Brazil
Cluster Analysis
DNA Fingerprinting
Food Microbiology
Genetic Variation
Genotype
Humans
Molecular Epidemiology
Molecular Sequence Data
Molecular Typing
Mycological Typing Techniques
Phylogeography
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
topic_facet animal feeds
feed-borne A. fumigatus
genetic variability estimation
molecular typing
molecular marker
Argentina
Article
Aspergillus fumigatus
Brazil
fungal biomass
fungal strain
genetic similarity
genetic variability
geographic origin
nonhuman
nucleotide sequence
polymerase chain reaction
random amplified polymorphic DNA
restriction fragment length polymorphism
animal
aspergillosis
Aspergillus fumigatus
classification
cluster analysis
DNA fingerprinting
DNA sequence
food control
genetic variation
genotype
human
isolation and purification
microbiological examination
microbiology
molecular epidemiology
molecular genetics
molecular typing
phylogeography
veterinary
Animals
Argentina
Aspergillosis
Aspergillus fumigatus
Brazil
Cluster Analysis
DNA Fingerprinting
Food Microbiology
Genetic Variation
Genotype
Humans
Molecular Epidemiology
Molecular Sequence Data
Molecular Typing
Mycological Typing Techniques
Phylogeography
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
description Aspergillus fumigatus, the major etiological agent of human and animal aspergillosis, is a toxigenic fungus largely regarded as a single species by macroscopic and microscopic features. However, molecular studies have demonstrated that several morphologically identified A. fumigatus strains might be genetically distinct. This work was aimed to apply PCR-restriction length fragment polymorphisms (PCR-RFLP) and random amplification of polymorphic DNA (RAPD) molecular markers to characterize a set of feed-borne and clinical A. fumigatus sensu lato strains isolated from Argentina and Brazil and to determine and compare their genetic variability. All A. fumigatus strains had the same band profile and those typical of A. fumigatus sensu stricto positive controls by PCR-RFLP. Moreover, all Argentinian and Brazilian strains typified by RAPD showed similar band patterns to each other and to A. fumigatus sensu stricto reference strains regardless of their isolation source (animal feeds or human/animal clinical cases) and geographic origin. Genetic similarity coefficients ranged from 0.61 to 1.00, but almost all isolates showed 78% of genetic similarly suggesting that genetic variability was found at intraspecific level. Finally, benA sequencing confirmed its identification as A. fumigatus sensu stricto species. These results suggest that A. fumigatus sensu stricto is a predominant species into Aspergillus section Fumigati found in animal environments as well as in human/animal clinical cases, while other species may be rarely isolated. The strains involved in human and animal aspergillosis could come from the environment where this fungus is frequently found. Rural workers and animals would be constantly exposed. © 2015 The Author.
title Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_short Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_full Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_fullStr Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_full_unstemmed Characterization and genetic variability of feed-borne and clinical animal/human Aspergillus fumigatus strains using molecular markers
title_sort characterization and genetic variability of feed-borne and clinical animal/human aspergillus fumigatus strains using molecular markers
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13693786_v53_n7_p699_Pena
http://hdl.handle.net/20.500.12110/paper_13693786_v53_n7_p699_Pena
_version_ 1768541766260097024