Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes

Aims: To study the influence of peptides and proteolytic enzymes in the osmotic adaptation of Lactobacillus casei. Methods and Results: Di- and tri-peptides added individually increased the osmotolerance of Lact. casei when grown in a chemically defined medium (CDM) containing NaCl. Growth stimulati...

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Autores principales:	Piuri, Mariana, Ruzal, Sandra Mónica
Publicado:	2003
Materias:	Lactobacillus casei Osmoregulation Peptides Proteolytic system carnitine peptide proline dipeptidase proline iminopeptidase protein PepI protein PepQ proteinase sodium chloride unclassified drug bacterium osmoregulation adaptation article bacterial growth culture medium DNA supercoiling enzyme activity gene repression genetic transcription Lactobacillus nonhuman protein degradation Culture Media Food Microbiology Genes, Bacterial Humans Osmolar Concentration Peptide Hydrolases Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases Sodium Chloride Transcription, Genetic Water-Electrolyte Balance Bacteria (microorganisms) Posibacteria
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v95_n2_p372_Piuri http://hdl.handle.net/20.500.12110/paper_13645072_v95_n2_p372_Piuri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_13645072_v95_n2_p372_Piuri
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spelling	paper:paper_13645072_v95_n2_p372_Piuri2023-06-08T16:11:47Z Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes Piuri, Mariana Ruzal, Sandra Mónica Lactobacillus casei Osmoregulation Peptides Proteolytic system carnitine peptide proline dipeptidase proline iminopeptidase protein PepI protein PepQ proteinase sodium chloride unclassified drug bacterium osmoregulation adaptation article bacterial growth culture medium DNA supercoiling enzyme activity gene repression genetic transcription Lactobacillus Lactobacillus casei nonhuman osmoregulation protein degradation Culture Media Food Microbiology Genes, Bacterial Humans Lactobacillus casei Osmolar Concentration Peptide Hydrolases Peptides Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases Sodium Chloride Transcription, Genetic Water-Electrolyte Balance Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria Aims: To study the influence of peptides and proteolytic enzymes in the osmotic adaptation of Lactobacillus casei. Methods and Results: Di- and tri-peptides added individually increased the osmotolerance of Lact. casei when grown in a chemically defined medium (CDM) containing NaCl. Growth stimulation and the re-establishment in their presence of plasmid DNA supercoiling (recovery of the linking number) in hyperosmotic medium indicated that they are used as osmocompatible solutes as carnithine a known osmoprotector does. The investigation of the proteolytic system showed that in high osmolarity medium, the cell envelope-associated proteinase (PrtP), and PepX (X-prolyl-dipeptidyl aminopeptidase) increased activity and lost repression by peptides. PepI, an iminopeptidase was also derepressed. PepQ, a prolidase that specifically liberated proline from dipeptides, was almost unaffected. Derepression in the presence of peptides took place at the transcriptional level. However, the twofold activation of PrtP in CDM hyperosmotic medium was essentially through an increase of the apparent Vmax of the enzyme. Conclusions: These results strongly suggest a contribution of the proteolytic system peptide supply in the osmotic adaptation. Significance and Impact of the Study: Advances in understanding the role of peptides in the adaptation to high osmolarity particularly involved in dairy processes. Fil:Piuri, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v95_n2_p372_Piuri http://hdl.handle.net/20.500.12110/paper_13645072_v95_n2_p372_Piuri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Lactobacillus casei Osmoregulation Peptides Proteolytic system carnitine peptide proline dipeptidase proline iminopeptidase protein PepI protein PepQ proteinase sodium chloride unclassified drug bacterium osmoregulation adaptation article bacterial growth culture medium DNA supercoiling enzyme activity gene repression genetic transcription Lactobacillus Lactobacillus casei nonhuman osmoregulation protein degradation Culture Media Food Microbiology Genes, Bacterial Humans Lactobacillus casei Osmolar Concentration Peptide Hydrolases Peptides Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases Sodium Chloride Transcription, Genetic Water-Electrolyte Balance Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria
spellingShingle	Lactobacillus casei Osmoregulation Peptides Proteolytic system carnitine peptide proline dipeptidase proline iminopeptidase protein PepI protein PepQ proteinase sodium chloride unclassified drug bacterium osmoregulation adaptation article bacterial growth culture medium DNA supercoiling enzyme activity gene repression genetic transcription Lactobacillus Lactobacillus casei nonhuman osmoregulation protein degradation Culture Media Food Microbiology Genes, Bacterial Humans Lactobacillus casei Osmolar Concentration Peptide Hydrolases Peptides Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases Sodium Chloride Transcription, Genetic Water-Electrolyte Balance Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria Piuri, Mariana Ruzal, Sandra Mónica Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
topic_facet	Lactobacillus casei Osmoregulation Peptides Proteolytic system carnitine peptide proline dipeptidase proline iminopeptidase protein PepI protein PepQ proteinase sodium chloride unclassified drug bacterium osmoregulation adaptation article bacterial growth culture medium DNA supercoiling enzyme activity gene repression genetic transcription Lactobacillus Lactobacillus casei nonhuman osmoregulation protein degradation Culture Media Food Microbiology Genes, Bacterial Humans Lactobacillus casei Osmolar Concentration Peptide Hydrolases Peptides Reverse Transcriptase Polymerase Chain Reaction Serine Endopeptidases Sodium Chloride Transcription, Genetic Water-Electrolyte Balance Bacteria (microorganisms) Lactobacillus Lactobacillus casei Posibacteria
description	Aims: To study the influence of peptides and proteolytic enzymes in the osmotic adaptation of Lactobacillus casei. Methods and Results: Di- and tri-peptides added individually increased the osmotolerance of Lact. casei when grown in a chemically defined medium (CDM) containing NaCl. Growth stimulation and the re-establishment in their presence of plasmid DNA supercoiling (recovery of the linking number) in hyperosmotic medium indicated that they are used as osmocompatible solutes as carnithine a known osmoprotector does. The investigation of the proteolytic system showed that in high osmolarity medium, the cell envelope-associated proteinase (PrtP), and PepX (X-prolyl-dipeptidyl aminopeptidase) increased activity and lost repression by peptides. PepI, an iminopeptidase was also derepressed. PepQ, a prolidase that specifically liberated proline from dipeptides, was almost unaffected. Derepression in the presence of peptides took place at the transcriptional level. However, the twofold activation of PrtP in CDM hyperosmotic medium was essentially through an increase of the apparent Vmax of the enzyme. Conclusions: These results strongly suggest a contribution of the proteolytic system peptide supply in the osmotic adaptation. Significance and Impact of the Study: Advances in understanding the role of peptides in the adaptation to high osmolarity particularly involved in dairy processes.
author	Piuri, Mariana Ruzal, Sandra Mónica
author_facet	Piuri, Mariana Ruzal, Sandra Mónica
author_sort	Piuri, Mariana
title	Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
title_short	Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
title_full	Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
title_fullStr	Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
title_full_unstemmed	Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes
title_sort	adaptation to high salt in lactobacillus: role of peptides and proteolytic enzymes
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13645072_v95_n2_p372_Piuri http://hdl.handle.net/20.500.12110/paper_13645072_v95_n2_p372_Piuri
work_keys_str_mv	AT piurimariana adaptationtohighsaltinlactobacillusroleofpeptidesandproteolyticenzymes AT ruzalsandramonica adaptationtohighsaltinlactobacillusroleofpeptidesandproteolyticenzymes
_version_	1768544745849618432

Adaptation to high salt in Lactobacillus: Role of peptides and proteolytic enzymes

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