Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa

Solar UVA radiation is one of the main environmental stress factors for Pseudomonas aeruginosa. Exposure to high UVA doses produces lethal effects by the action of the reactive oxygen species (ROS) it generates. P. aeruginosa has several enzymes, including KatA and KatB catalases, which provide deto...

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Autores principales:	Tribelli, Paula María, Costa, Cristina Susana
Publicado:	2016
Materias:	beta galactosidase catalase drinking water enzyme hydrogen peroxide hypochlorite sodium KatA enzyme KatB enzyme messenger RNA polyacrylamide gel reactive oxygen metabolite unclassified drug oxidizing agent adaptation Article bacterial growth chemoluminescence disinfection enzyme activity native polyacrylamide gel electrophoresis nonhuman oxidative stress priority journal Pseudomonas aeruginosa radiation dose real time polymerase chain reaction solar radiation ultraviolet A radiation gene expression regulation genetics metabolism oxidation reduction reaction physiology radiation response ultraviolet radiation Adaptation, Physiological Catalase Gene Expression Regulation, Bacterial Hydrogen Peroxide Oxidants Oxidation-Reduction Oxidative Stress Sodium Hypochlorite Ultraviolet Rays
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v162_n5_p855_Pezzoni http://hdl.handle.net/20.500.12110/paper_13500872_v162_n5_p855_Pezzoni
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_13500872_v162_n5_p855_Pezzoni2023-06-08T16:10:47Z Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa Tribelli, Paula María Costa, Cristina Susana beta galactosidase catalase drinking water enzyme hydrogen peroxide hypochlorite sodium KatA enzyme KatB enzyme messenger RNA polyacrylamide gel reactive oxygen metabolite unclassified drug catalase hydrogen peroxide hypochlorite sodium oxidizing agent adaptation Article bacterial growth chemoluminescence disinfection enzyme activity native polyacrylamide gel electrophoresis nonhuman oxidative stress priority journal Pseudomonas aeruginosa radiation dose real time polymerase chain reaction solar radiation ultraviolet A radiation gene expression regulation genetics metabolism oxidation reduction reaction physiology Pseudomonas aeruginosa radiation response ultraviolet radiation Adaptation, Physiological Catalase Gene Expression Regulation, Bacterial Hydrogen Peroxide Oxidants Oxidation-Reduction Oxidative Stress Pseudomonas aeruginosa Sodium Hypochlorite Ultraviolet Rays Solar UVA radiation is one of the main environmental stress factors for Pseudomonas aeruginosa. Exposure to high UVA doses produces lethal effects by the action of the reactive oxygen species (ROS) it generates. P. aeruginosa has several enzymes, including KatA and KatB catalases, which provide detoxification of ROS. We have previously demonstrated that KatA is essential in defending P. aeruginosa against high UVA doses. In order to analyse the mechanisms involved in the adaptation of this micro-organism to UVA, we investigated the effect of exposure to low UVA doses on KatA and KatB activities, and the physiological consequences. Exposure to UVA induced total catalase activity; assays with non-denaturing polyacrylamide gels showed that both KatA and KatB activities were increased by radiation. This regulation occurred at the transcriptional level and depended, at least partly, on the increase in H2O2 levels. We demonstrated that exposure to low UVA produced a protective effect against subsequent lethal doses of UVA, sodium hypochlorite and H2O2. Protection against lethal UVA depends on katA, whilst protection against sodium hypochlorite depends on katB, demonstrating that different mechanisms are involved in the defence against these oxidative agents, although both genes can be involved in the global cellular response. Conversely, protection against lethal doses of H2O2 could depend on induction of both genes and/or (an)other defensive factor(s). A better understanding of the adaptive response of P. aeruginosa to UVA is relevant from an ecological standpoint and for improving disinfection strategies that employ UVA or solar irradiation. © 2016 The Authors. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Costa, C.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v162_n5_p855_Pezzoni http://hdl.handle.net/20.500.12110/paper_13500872_v162_n5_p855_Pezzoni
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	beta galactosidase catalase drinking water enzyme hydrogen peroxide hypochlorite sodium KatA enzyme KatB enzyme messenger RNA polyacrylamide gel reactive oxygen metabolite unclassified drug catalase hydrogen peroxide hypochlorite sodium oxidizing agent adaptation Article bacterial growth chemoluminescence disinfection enzyme activity native polyacrylamide gel electrophoresis nonhuman oxidative stress priority journal Pseudomonas aeruginosa radiation dose real time polymerase chain reaction solar radiation ultraviolet A radiation gene expression regulation genetics metabolism oxidation reduction reaction physiology Pseudomonas aeruginosa radiation response ultraviolet radiation Adaptation, Physiological Catalase Gene Expression Regulation, Bacterial Hydrogen Peroxide Oxidants Oxidation-Reduction Oxidative Stress Pseudomonas aeruginosa Sodium Hypochlorite Ultraviolet Rays
spellingShingle	beta galactosidase catalase drinking water enzyme hydrogen peroxide hypochlorite sodium KatA enzyme KatB enzyme messenger RNA polyacrylamide gel reactive oxygen metabolite unclassified drug catalase hydrogen peroxide hypochlorite sodium oxidizing agent adaptation Article bacterial growth chemoluminescence disinfection enzyme activity native polyacrylamide gel electrophoresis nonhuman oxidative stress priority journal Pseudomonas aeruginosa radiation dose real time polymerase chain reaction solar radiation ultraviolet A radiation gene expression regulation genetics metabolism oxidation reduction reaction physiology Pseudomonas aeruginosa radiation response ultraviolet radiation Adaptation, Physiological Catalase Gene Expression Regulation, Bacterial Hydrogen Peroxide Oxidants Oxidation-Reduction Oxidative Stress Pseudomonas aeruginosa Sodium Hypochlorite Ultraviolet Rays Tribelli, Paula María Costa, Cristina Susana Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
topic_facet	beta galactosidase catalase drinking water enzyme hydrogen peroxide hypochlorite sodium KatA enzyme KatB enzyme messenger RNA polyacrylamide gel reactive oxygen metabolite unclassified drug catalase hydrogen peroxide hypochlorite sodium oxidizing agent adaptation Article bacterial growth chemoluminescence disinfection enzyme activity native polyacrylamide gel electrophoresis nonhuman oxidative stress priority journal Pseudomonas aeruginosa radiation dose real time polymerase chain reaction solar radiation ultraviolet A radiation gene expression regulation genetics metabolism oxidation reduction reaction physiology Pseudomonas aeruginosa radiation response ultraviolet radiation Adaptation, Physiological Catalase Gene Expression Regulation, Bacterial Hydrogen Peroxide Oxidants Oxidation-Reduction Oxidative Stress Pseudomonas aeruginosa Sodium Hypochlorite Ultraviolet Rays
description	Solar UVA radiation is one of the main environmental stress factors for Pseudomonas aeruginosa. Exposure to high UVA doses produces lethal effects by the action of the reactive oxygen species (ROS) it generates. P. aeruginosa has several enzymes, including KatA and KatB catalases, which provide detoxification of ROS. We have previously demonstrated that KatA is essential in defending P. aeruginosa against high UVA doses. In order to analyse the mechanisms involved in the adaptation of this micro-organism to UVA, we investigated the effect of exposure to low UVA doses on KatA and KatB activities, and the physiological consequences. Exposure to UVA induced total catalase activity; assays with non-denaturing polyacrylamide gels showed that both KatA and KatB activities were increased by radiation. This regulation occurred at the transcriptional level and depended, at least partly, on the increase in H2O2 levels. We demonstrated that exposure to low UVA produced a protective effect against subsequent lethal doses of UVA, sodium hypochlorite and H2O2. Protection against lethal UVA depends on katA, whilst protection against sodium hypochlorite depends on katB, demonstrating that different mechanisms are involved in the defence against these oxidative agents, although both genes can be involved in the global cellular response. Conversely, protection against lethal doses of H2O2 could depend on induction of both genes and/or (an)other defensive factor(s). A better understanding of the adaptive response of P. aeruginosa to UVA is relevant from an ecological standpoint and for improving disinfection strategies that employ UVA or solar irradiation. © 2016 The Authors.
author	Tribelli, Paula María Costa, Cristina Susana
author_facet	Tribelli, Paula María Costa, Cristina Susana
author_sort	Tribelli, Paula María
title	Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
title_short	Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
title_full	Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
title_fullStr	Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
title_full_unstemmed	Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
title_sort	exposure to low uva doses increases kata and katb catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_13500872_v162_n5_p855_Pezzoni http://hdl.handle.net/20.500.12110/paper_13500872_v162_n5_p855_Pezzoni
work_keys_str_mv	AT tribellipaulamaria exposuretolowuvadosesincreaseskataandkatbcatalaseactivitiesandconferscrossprotectionagainstsubsequentoxidativeinjuriesinpseudomonasaeruginosa AT costacristinasusana exposuretolowuvadosesincreaseskataandkatbcatalaseactivitiesandconferscrossprotectionagainstsubsequentoxidativeinjuriesinpseudomonasaeruginosa
_version_	1768541669656887296

Exposure to low UVA doses increases KatA and KatB catalase activities, and confers cross-protection against subsequent oxidative injuries in pseudomonas aeruginosa

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